HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 28, 26321-26329, July 15, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/28/26321    most recent M413993200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alder-Baerens, N. Articles by Herrmann, A. Search for Related Content PubMed PubMed Citation Articles by Alder-Baerens, N. Articles by Herrmann, A. Social Bookmarking                      What's this?

Headgroup-specific Exposure of Phospholipids in ABCA1-expressing Cells^*

Nele Alder-Baerens, Peter Müller¶, Antje Pohl, Thomas Korte, Yannick Hamon||, Giovanna Chimini||**, Thomas Pomorski¶, and Andreas Herrmann**

From the Humboldt University of Berlin, Institute of Biology, Center of Biophysics and Bioinformatics, Invalidenstrasse 42, 10115 Berlin, Germany and the ^||Centre d'Immunologie de Marseille Luminy, Case 906, Parc Scientifique de Luminy, 13288 Marseille Cedex 09, France

ABCA1 has been established to be required for the efflux of cholesterol and phospholipids to apolipoproteins such as apoA-I. At present, it is unclear whether ABCA1-mediated lipid exposure is specific with regard to lipid headgroups and whether it requires calcium activation and the presence of a lipid acceptor. In the present work, we found exofacial exposure of endogenous phosphatidylserine in the absence of apoA-I to be enhanced in ABCA1-GFP expressing MDCKII and HeLa cells compared with control cells. By using C6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD)-labeled phospholipid analogues, we observed elevated redistribution of phosphatidylserine and phosphatidylethanolamine but not of phosphatidylcholine analogues from the cytoplasmic to the exoplasmic leaflet of the plasma membrane of ABCA1-GFP expressing cells. Whereas glyburide affected neither the level of exofacial endogenous PS nor the outward movement of the amino phospholipid analogues, the latter was sensitive to intracellular Ca²⁺ in ABCA1-GFP expressing cells, further enhancing outward analogue redistribution with respect to control cells. Both receptor-mediated endocytosis and fluidphase endocytosis were reduced in MDCKII cells expressing ABCA1-GFP. Glyburide raised the level of receptor-mediated endocytosis in the ABCA1-GFP expressing cell to the level of control cells in the absence of glyburide. In control cells, however, fluid-phase endocytosis but not receptor-mediated endocytosis was significantly reduced upon glyburide treatment.

Received for publication, December 13, 2004 , and in revised form, May 17, 2005.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Supported by a fellowship of the Schering Foundation.

^¶ Supported by a grant from the Deutsche Forschungsgemeinschaft.

^** Supported by European Union Grant MRTN-CT-2004-005330.

To whom correspondence should be addressed: Humboldt University of Berlin, Institute of Biology, Molecular Biophysics, Invalidenstrasse 42, 10115 Berlin, Germany. Tel.: 49-30-2093-8830; Fax: 49-302093-8585; E-mail: andreas.herrmann{at}rz.hu-berlin.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. M. Argraves and W. S. Argraves HDL serves as a S1P signaling platform mediating a multitude of cardiovascular effects J. Lipid Res., November 1, 2007; 48(11): 2325 - 2333. [Abstract] [Full Text] [PDF]

				M. Pennings, R. B. Hildebrand, D. Ye, C. Kunne, T. J.C. Van Berkel, A. K. Groen, and M. Van Eck Bone marrow-derived multidrug resistance protein ABCB4 protects against atherosclerotic lesion development in LDL receptor knockout mice Cardiovasc Res, October 1, 2007; 76(1): 175 - 183. [Abstract] [Full Text] [PDF]

				C. Vedhachalam, P. T. Duong, M. Nickel, D. Nguyen, P. Dhanasekaran, H. Saito, G. H. Rothblat, S. Lund-Katz, and M. C. Phillips Mechanism of ATP-binding Cassette Transporter A1-mediated Cellular Lipid Efflux to Apolipoprotein A-I and Formation of High Density Lipoprotein Particles J. Biol. Chem., August 24, 2007; 282(34): 25123 - 25130. [Abstract] [Full Text] [PDF]

				D. L. Daleke Phospholipid Flippases J. Biol. Chem., January 12, 2007; 282(2): 821 - 825. [Full Text] [PDF]

				Y. D. Landry, M. Denis, S. Nandi, S. Bell, A. M. Vaughan, and X. Zha ATP-binding Cassette Transporter A1 Expression Disrupts Raft Membrane Microdomains through Its ATPase-related Functions J. Biol. Chem., November 24, 2006; 281(47): 36091 - 36101. [Abstract] [Full Text] [PDF]

				P. T. Duong, H. L. Collins, M. Nickel, S. Lund-Katz, G. H. Rothblat, and M. C. Phillips Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I J. Lipid Res., April 1, 2006; 47(4): 832 - 843. [Abstract] [Full Text] [PDF]