Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M413312200 on May 16, 2005

J. Biol. Chem., Vol. 280, Issue 28, 26330-26338, July 15, 2005
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
280/28/26330    most recent
M413312200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robenek, H.
Right arrow Articles by Severs, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robenek, H.
Right arrow Articles by Severs, N. J.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains*

Horst Robenek{ddagger}§, Mirko J. Robenek{ddagger}, Insa Buers{ddagger}, Stefan Lorkowski{ddagger}, Oliver Hofnagel{ddagger}, David Troyer{ddagger}, and Nicholas J. Severs¶

From the {ddagger}Leibniz-Institute for Arteriosclerosis Research, University of Münster, Münster, Germany and the National Heart and Lung Institute, Imperial College, London SW3 6LY, United Kingdom

Proteins of the PAT family, named after perilipin, adipophilin, and TIP47 (tail-interacting protein of 47 kDa), are associated with lipid droplets and have previously been localized by immunofluorescence microscopy exclusively to the droplet surface. These proteins are considered not to be present in any other subcellular compartment. By applying the high resolution technique of freeze-fracture electron microscopy combined with immunogold labeling, we now demonstrate that in macrophages and adipocytes PAT family proteins are, first, distributed not only in the surface but also throughout the lipid droplet core and, second, are integral components of the plasma membrane. Under normal culture conditions these proteins are dispersed in the cytoplasmic leaflet of the plasma membrane. Stimulation of lipid droplet formation by incubation of the cells with acetylated low density lipoprotein leads to clustering of the PAT family proteins in raised plasma membrane domains. Fractures penetrating beneath the plasma membrane demonstrate that lipid droplets are closely apposed to these domains. A similar distribution pattern of labeling in the form of linear aggregates within the clusters is apparent in the cytoplasmic monolayer of the plasma membrane and the immediately adjacent outer monolayer of the lipid droplet. The aggregation of the PAT family proteins into such assemblies may facilitate carrier-mediated lipid influx from the extracellular environment into the lipid droplet. Lipid droplets appear to acquire their PAT proteins by interaction with plasma membrane domains enriched in these proteins.


Received for publication, November 26, 2004 , and in revised form, April 27, 2005.

* This work was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 492. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Cell Biology and Ultrastructure Research, Leibniz-Institute for Arteriosclerosis Research, University of Münster, Domagkstr. 3, 48149 Münster, Germany. Tel.: 49-251-835-6426; Fax: 49-251-835-2998; E-mail: robenek{at}uni-muenster.de.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
EndocrinologyHome page
J. Borg, C. Klint, N. Wierup, K. Strom, S. Larsson, F. Sundler, R. Lupi, P. Marchetti, G. Xu, A. Kimmel, et al.
Perilipin Is Present in Islets of Langerhans and Protects against Lipotoxicity When Overexpressed in the {beta}-Cell Line INS-1
Endocrinology, July 1, 2009; 150(7): 3049 - 3057.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
I. Buers, H. Robenek, S. Lorkowski, Y. Nitschke, N. J. Severs, and O. Hofnagel
TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism
Arterioscler. Thromb. Vasc. Biol., May 1, 2009; 29(5): 767 - 773.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
I. Barba, L. Chavarria, M. Ruiz-Meana, M. Mirabet, E. Agullo, and D. Garcia-Dorado
Effect of intracellular lipid droplets on cytosolic Ca2+ and cell death during ischaemia-reperfusion injury in cardiomyocytes
J. Physiol., March 15, 2009; 587(6): 1331 - 1341.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
G. Murphy Jr., R. L. Rouse, W. W. Polk, W. G. Henk, S. A. Barker, M. J. Boudreaux, Z. E. Floyd, and A. L. Penn
Combustion-Derived Hydrocarbons Localize to Lipid Droplets in Respiratory Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 532 - 540.
[Abstract] [Full Text] [PDF]


Home page
Mol. Pharmacol.Home page
G. Muller, S. Wied, N. Walz, and C. Jung
Translocation of Glycosylphosphatidylinositol-Anchored Proteins from Plasma Membrane Microdomains to Lipid Droplets in Rat Adipocytes Is Induced by Palmitate, H2O2, and the Sulfonylurea Drug Glimepiride
Mol. Pharmacol., May 1, 2008; 73(5): 1513 - 1529.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. M. Maya-Monteiro, P. E. Almeida, H. D'Avila, A. S. Martins, A. P. Rezende, H. Castro-Faria-Neto, and P. T. Bozza
Leptin Induces Macrophage Lipid Body Formation by a Phosphatidylinositol 3-Kinase- and Mammalian Target of Rapamycin-dependent Mechanism
J. Biol. Chem., January 25, 2008; 283(4): 2203 - 2210.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Renal Physiol.Home page
J. Dominguez, P. Wu, C. S. Packer, C. Temm, and K. J. Kelly
Lipotoxic and inflammatory phenotypes in rats with uncontrolled metabolic syndrome and nephropathy
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F670 - F679.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
S. Boulant, P. Targett-Adams, and J. McLauchlan
Disrupting the association of hepatitis C virus core protein with lipid droplets correlates with a loss in production of infectious virus
J. Gen. Virol., August 1, 2007; 88(8): 2204 - 2213.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
K. Jaworski, E. Sarkadi-Nagy, R. E. Duncan, M. Ahmadian, and H. S. Sul
Regulation of Triglyceride Metabolism. * IV. Hormonal regulation of lipolysis in adipose tissue
Am J Physiol Gastrointest Liver Physiol, July 1, 2007; 293(1): G1 - G4.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
H. Robenek, O. Hofnagel, I. Buers, M. J. Robenek, D. Troyer, and N. J. Severs
Adipophilin-enriched domains in the ER membrane are sites of lipid droplet biogenesis
J. Cell Sci., October 15, 2006; 119(20): 4215 - 4224.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
S. Lopez-Verges, G. Camus, G. Blot, R. Beauvoir, R. Benarous, and C. Berlioz-Torrent
Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions
PNAS, October 3, 2006; 103(40): 14947 - 14952.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
H. Robenek, O. Hofnagel, I. Buers, S. Lorkowski, M. Schnoor, M. J. Robenek, H. Heid, D. Troyer, and N. J. Severs
Butyrophilin controls milk fat globule secretion
PNAS, July 5, 2006; 103(27): 10385 - 10390.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Yamaguchi, S. Matsushita, K. Motojima, F. Hirose, and T. Osumi
MLDP, a Novel PAT Family Protein Localized to Lipid Droplets and Enriched in the Heart, Is Regulated by Peroxisome Proliferator-activated Receptor {alpha}
J. Biol. Chem., May 19, 2006; 281(20): 14232 - 14240.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. Aboulaich, A. V. Vener, and P. Stralfors
Hormonal Control of Reversible Translocation of Perilipin B to the Plasma Membrane in Primary Human Adipocytes
J. Biol. Chem., April 28, 2006; 281(17): 11446 - 11449.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
P. Kleinbongard, R. Schulz, T. Rassaf, T. Lauer, A. Dejam, T. Jax, I. Kumara, P. Gharini, S. Kabanova, B. Ozuyaman, et al.
Red blood cells express a functional endothelial nitric oxide synthase
Blood, April 1, 2006; 107(7): 2943 - 2951.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
B. H.-J. Chang, L. Li, A. Paul, S. Taniguchi, V. Nannegari, W. C. Heird, and L. Chan
Protection against Fatty Liver but Normal Adipogenesis in Mice Lacking Adipose Differentiation-Related Protein
Mol. Cell. Biol., February 1, 2006; 26(3): 1063 - 1076.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement