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J. Biol. Chem., Vol. 280, Issue 28, 26557-26564, July 15, 2005

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Identification of a Myosin VII-Talin Complex^*

Richard I. Tuxworth, Stephen Stephens, Zachary C. Ryan, and Margaret A. Titus

From the Department of Genetics, Cell Biology, and Development, the University of Minnesota, Minneapolis, Minnesota 55455

Myosin VII (M7) plays a role in adhesion in both Dictyostelium and mammalian cells where it is a component of a complex of proteins that serve to link membrane receptors to the underlying actin cytoskeleton. The nature of this complex is not fully known, prompting a search for M7-binding proteins. Co-immunoprecipitation experiments reveal that Dictyostelium M7 (DdM7) interacts with talinA, an actin-binding protein with a known role in cell-substrate adhesion. No additional proteins are observed in the immunoprecipitate, indicating that the interaction is direct. The N-terminal region of the DdM7 tail that lies between the region of predicted coil and the first MyTH4 domain is found to harbor the talinA binding site. Localization experiments reveal that talinA does not serve as a membrane receptor for DdM7 and vice versa. These findings reveal that talinA is a major DdM7 binding partner and suggest that their interaction induces a conformational change in each that, in combination with membrane receptor binding, promotes the assembly of a high avidity receptor complex essential for adhesion of the cell to substrata.

Received for publication, April 5, 2005

^* This work was supported by a Human Frontier Science Program Long Term Fellowship (to R. I. T.) and National Institutes of Health Grant GM046486 (to M. A. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Current address: Medical Research Council Centre for Developmental Neurobiology, New Hunt's House, Kings College London, Guy's Campus, London SE1 1UL, United Kingdom.

To whom correspondence should be addressed: Dept. of Genetics, Cell Biology & Development, 6-160 Jackson Hall, 321 Church St. SE, University of Minnesota, Minneapolis, MN 55455. Tel.: 612-625-8498; Fax: 612-625-4648; E-mail: titus{at}lenti.med.umn.edu.

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