HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 29, 26813-26824, July 22, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/29/26813    most recent M503576200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zeenko, V. Articles by Gallie, D. R. Search for Related Content PubMed PubMed Citation Articles by Zeenko, V. Articles by Gallie, D. R. Social Bookmarking                      What's this?

Cap-independent Translation of Tobacco Etch Virus Is Conferred by an RNA Pseudoknot in the 5'-Leader^*

Vladimir Zeenko and Daniel R. Gallie

From the Department of Biochemistry, University of California, Riverside, California 92521-0129

The tobacco etch virus (TEV) 5'-leader promotes cap-independent translation in a 5'-proximal position and promotes internal initiation when present in the intercistronic region of a dicistronic mRNA, indicating that the leader contains an internal ribosome entry site. The TEV 143-nucleotide 5'-leader folds into a structure that contains two domains, each of which contains an RNA pseudoknot. Mutational analysis of the TEV 5'-leader identified pseudoknot (PK) 1 within the 5'-proximal domain and an upstream single-stranded region flanking PK1 as necessary to promote cap-independent translation. Mutations to either stem or to loops 2 or 3 of PK1 substantially disrupted cap-independent translation. The sequence of loop 3 in PK1 is complementary to a region in 18 S rRNA that is conserved throughout eukaryotes. Mutations within L3 that disrupted its potential base pairing with 18 S rRNA reduced cap-independent translation, whereas mutations that maintained the potential for base pairing with 18 S rRNA had little effect. These results indicated that the TEV 5'-leader functionally substitutes for a 5'-cap and promotes cap-independent translation through a 45-nucleotide pseudoknot-containing domain.

Received for publication, April 1, 2005 , and in revised form, May 19, 2005.

^* This work was supported by Grant MCB-0130664 from the National Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Biochemistry, University of California, Riverside, CA 92521-0129. Tel.: 951-827-7298; Fax: 951-827-4434; E-mail: drgallie{at}citrus.ucr.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. A. Khan, H. Miyoshi, D. R. Gallie, and D. J. Goss Potyvirus Genome-linked Protein, VPg, Directly Affects Wheat Germ in Vitro Translation: INTERACTIONS WITH TRANSLATION INITIATION FACTORS eIF4F AND eIFiso4F J. Biol. Chem., January 18, 2008; 283(3): 1340 - 1349. [Abstract] [Full Text] [PDF]

				A. Karetnikov and K. Lehto The RNA2 5' leader of Blackcurrant reversion virus mediates efficient in vivo translation through an internal ribosomal entry site mechanism J. Gen. Virol., January 1, 2007; 88(1): 286 - 297. [Abstract] [Full Text] [PDF]

				S. Ray, H. Yumak, A. Domashevskiy, M. A. Khan, D. R. Gallie, and D. J. Goss Tobacco Etch Virus mRNA Preferentially Binds Wheat Germ Eukaryotic Initiation Factor (eIF) 4G Rather than eIFiso4G J. Biol. Chem., November 24, 2006; 281(47): 35826 - 35834. [Abstract] [Full Text] [PDF]

				M. A. Khan, H. Miyoshi, S. Ray, T. Natsuaki, N. Suehiro, and D. J. Goss Interaction of Genome-linked Protein (VPg) of Turnip Mosaic Virus with Wheat Germ Translation Initiation Factors eIFiso4E and eIFiso4F J. Biol. Chem., September 22, 2006; 281(38): 28002 - 28010. [Abstract] [Full Text] [PDF]