HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 3, 1921-1930, January 21, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/3/1921    most recent M403632200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kiel, J. A. K. W. Articles by Kunau, W.-H. Search for Related Content PubMed PubMed Citation Articles by Kiel, J. A. K. W. Articles by Kunau, W.-H. Social Bookmarking                      What's this?

Ubiquitination of the Peroxisomal Targeting Signal Type 1 Receptor, Pex5p, Suggests the Presence of a Quality Control Mechanism during Peroxisomal Matrix Protein Import^*

Jan A. K. W. Kiel, Kerstin Emmrich¶, Helmut E. Meyer||, and Wolf-H. Kunau¶

From the Abteilung für Zellbiochemie and the ^||Medical Proteom Center, Medizinische Fakultät der Ruhr-Universität Bochum, D-44780 Bochum, Germany

PEX genes encode proteins (peroxins) that are required for the biogenesis of peroxisomes. One of these peroxins, Pex5p, is the receptor for matrix proteins with a type 1 peroxisomal targeting signal (PTS1), which shuttles newly synthesized proteins from the cytosol into the peroxisome matrix. We observed that in various Saccharomyces cerevisiae pex mutants disturbed in the early stages of PTS1 import, the steady-state levels of Pex5p are enhanced relative to wild type controls. Furthermore, we identified ubiquitinated forms of Pex5p in deletion mutants of those PEX genes that have been implicated in recycling of Pex5p from the peroxisomal membrane into the cytosol. Pex5p ubiquitination required the presence of the ubiquitin-conjugating enzyme Ubc4p and the peroxins that are required during early stages of PTS1 protein import. Finally, we provide evidence that the proteasome is involved in the turnover of Pex5p in wild type yeast cells, a process that requires Ubc4p and occurs at the peroxisomal membrane. Our data suggest that during receptor recycling a portion of Pex5p becomes ubiquitinated and degraded by the proteasome. We propose that this process represents a conserved quality control mechanism in peroxisome biogenesis.

Received for publication, April 1, 2004 , and in revised form, October 27, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Supported by the Sonderforschungsbereiche (Grant SFB480/Teil-projekt B1) and by the Deutsche Forschungs Gemeinschaft (DFG Grant Ku 329/17-5).

Supported by the Sonderforschungsbereiche (Grants SFB394 and SFB480). To whom correspondence should be addressed (present address): Eukaryotic Microbiology, GBB, University of Groningen, Kerklaan 30 NL-9751 NN Haren, The Netherlands. Tel.: 31-50-363-2218; Fax: 31-50-363-2154; E-mail: J.A.K.W.Kiel{at}Biol.RUG.nl.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Rahim, S. Bischof, F. Kessler, and B. Agne In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system J. Exp. Bot., November 14, 2008; (2008) ern283v1. [Abstract] [Full Text] [PDF]

				C. P. Grou, A. F. Carvalho, M. P. Pinto, S. Wiese, H. Piechura, H. E. Meyer, B. Warscheid, C. Sa-Miranda, and J. E. Azevedo Members of the E2D (UbcH5) Family Mediate the Ubiquitination of the Conserved Cysteine of Pex5p, the Peroxisomal Import Receptor J. Biol. Chem., May 23, 2008; 283(21): 14190 - 14197. [Abstract] [Full Text] [PDF]

				A. F. Carvalho, M. P. Pinto, C. P. Grou, I. S. Alencastre, M. Fransen, C. Sa-Miranda, and J. E. Azevedo Ubiquitination of Mammalian Pex5p, the Peroxisomal Import Receptor J. Biol. Chem., October 26, 2007; 282(43): 31267 - 31272. [Abstract] [Full Text] [PDF]

				C. Williams, M. van den Berg, R. R. Sprenger, and B. Distel A Conserved Cysteine Is Essential for Pex4p-dependent Ubiquitination of the Peroxisomal Import Receptor Pex5p J. Biol. Chem., August 3, 2007; 282(31): 22534 - 22543. [Abstract] [Full Text] [PDF]

				H. W. Platta, F. E. Magraoui, D. Schlee, S. Grunau, W. Girzalsky, and R. Erdmann Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling J. Cell Biol., April 23, 2007; 177(2): 197 - 204. [Abstract] [Full Text] [PDF]

				S. Leon and S. Subramani A Conserved Cysteine Residue of Pichia pastoris Pex20p Is Essential for Its Recycling from the Peroxisome to the Cytosol J. Biol. Chem., March 9, 2007; 282(10): 7424 - 7430. [Abstract] [Full Text] [PDF]

				O. Panasenko, E. Landrieux, M. Feuermann, A. Finka, N. Paquet, and M. A. Collart The Yeast Ccr4-Not Complex Controls Ubiquitination of the Nascent-associated Polypeptide (NAC-EGD) Complex J. Biol. Chem., October 20, 2006; 281(42): 31389 - 31398. [Abstract] [Full Text] [PDF]

				A. Schluter, S. Fourcade, R. Ripp, J. L. Mandel, O. Poch, and A. Pujol The Evolutionary Origin of Peroxisomes: An ER-Peroxisome Connection Mol. Biol. Evol., April 1, 2006; 23(4): 838 - 845. [Abstract] [Full Text] [PDF]

				A. van der Zand, I. Braakman, H. J. Geuze, and H. F. Tabak The return of the peroxisome. J. Cell Sci., March 15, 2006; 119(Pt 6): 989 - 994. [Abstract] [Full Text] [PDF]

				S. Leon, L. Zhang, W. H. McDonald, J. Yates III, J. M. Cregg, and S. Subramani Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation J. Cell Biol., January 3, 2006; 172(1): 67 - 78. [Abstract] [Full Text] [PDF]

				B. K. Zolman, M. Monroe-Augustus, I. D. Silva, and B. Bartel Identification and Functional Characterization of Arabidopsis PEROXIN4 and the Interacting Protein PEROXIN22 PLANT CELL, December 1, 2005; 17(12): 3422 - 3435. [Abstract] [Full Text] [PDF]

				A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel The Saccharomyces cerevisiae Peroxisomal Import Receptor Pex5p Is Monoubiquitinated in Wild Type Cells J. Biol. Chem., March 4, 2005; 280(9): 7867 - 7874. [Abstract] [Full Text] [PDF]