HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 30, 27552-27560, July 29, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/30/27552    most recent M502422200v2 M502422200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lefevre, P. Articles by Bonifer, C. Search for Related Content PubMed PubMed Citation Articles by Lefevre, P. Articles by Bonifer, C. Social Bookmarking                      What's this?

Differentiation-dependent Alterations in Histone Methylation and Chromatin Architecture at the Inducible Chicken Lysozyme Gene^*

Pascal Lefevre, Claire Lacroix, Hiromi Tagoh, Maarten Hoogenkamp, Svitlana Melnik, Richard Ingram, and Constanze Bonifer

From the Division of Experimental Haematology, University of Leeds, St. James's University Hospital, Clinical Sciences Building, Leeds LS97TF, United Kingdom

It is now well established that locus-wide chromatin remodeling and dynamic alterations of histone modifications are required for the developmentally regulated activation of tissue-specific genes. However, little is known about the dynamics of these events during cell differentiation and how chromatin of an entire gene locus responds to signal transduction processes. To address this issue we investigated chromatin accessibility, linker histone distribution, and the histone methylation status at the macrophage-specific chicken lysozyme locus and the ubiquitously expressed gas41 locus in multipotent precursor cell lines and BM2 monoblast cells. The latter can be induced to go through macrophage maturation by treatment with phorbol-12-myristate acetate and can be further stimulated with bacterial lipopolysaccharide. We show that expression of the lysozyme gene in undifferentiated monoblasts is low and that a high level of gene expression requires both cell differentiation and lipopolysaccharide stimulation. However, depletion of the linker histone H1 is observed already in lysozyme non-expressing multipotent precursor cells. In undifferentiated monoblasts, the lysozyme regulatory regions are marked by the presence of monomethylated histone H3 lysine 4, which becomes increasingly converted into trimethylated H3 lysine K4 during cell differentiation. We also present evidence for extensive, differentiation-dependent alterations in nuclease accessibility at the lysozyme promoter without alterations of nucleosome and transcription factor occupancy.

Received for publication, March 4, 2005 , and in revised form, May 26, 2005.

^* This work was funded in part by the Welcome Trust, the Biotechnological and Biological Sciences Research Council, the City of Hope Medical Centre, and the Leukemia Research Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Figs. S1 and S2 and Tables S1 and S2.

A Fellow of the Kay Kendall Leukemia Fund.

To whom correspondence should be addressed. Tel.: 44-113-206-5676; Fax: 44-113-244-475; E-mail: c.bonifer{at}leeds.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Hoogenkamp, M. Lichtinger, H. Krysinska, C. Lancrin, D. Clarke, A. Williamson, L. Mazzarella, R. Ingram, H. Jorgensen, A. Fisher, et al. Early chromatin unfolding by RUNX1: a molecular explanation for differential requirements during specification versus maintenance of the hematopoietic gene expression program Blood, July 9, 2009; 114(2): 299 - 309. [Abstract] [Full Text] [PDF]

				K. Walter, C. Bonifer, and H. Tagoh Stem cell-specific epigenetic priming and B cell-specific transcriptional activation at the mouse Cd19 locus Blood, September 1, 2008; 112(5): 1673 - 1682. [Abstract] [Full Text] [PDF]

				R. Ingram, C. Gao, J. LeBon, Q. Liu, R. J. Mayoral, S. S. Sommer, M. Hoogenkamp, A. D. Riggs, and C. Bonifer PAP-LMPCR for improved, allele-specific footprinting and automated chromatin fine structure analysis Nucleic Acids Res., February 11, 2008; 36(3): e19 - e19. [Abstract] [Full Text] [PDF]

				O. Ivanova, D. Braas, and K.-H. Klempnauer Oncogenic point mutations in the Myb DNA-binding domain alter the DNA-binding properties of Myb at a physiological target gene Nucleic Acids Res., December 18, 2007; 35(21): 7237 - 7247. [Abstract] [Full Text] [PDF]

				H. Yoshioka, C. B. Geyer, J. L. Hornecker, K. T. Patel, and J. R. McCarrey In Vivo Analysis of Developmentally and Evolutionarily Dynamic Protein-DNA Interactions Regulating Transcription of the Pgk2 Gene during Mammalian Spermatogenesis Mol. Cell. Biol., November 15, 2007; 27(22): 7871 - 7885. [Abstract] [Full Text] [PDF]

				M. Hoogenkamp, H. Krysinska, R. Ingram, G. Huang, R. Barlow, D. Clarke, A. Ebralidze, P. Zhang, H. Tagoh, P. N. Cockerill, et al. The Pu.1 Locus Is Differentially Regulated at the Level of Chromatin Structure and Noncoding Transcription by Alternate Mechanisms at Distinct Developmental Stages of Hematopoiesis Mol. Cell. Biol., November 1, 2007; 27(21): 7425 - 7438. [Abstract] [Full Text] [PDF]

				R. P. DeKoter, B. L. Schweitzer, M. B. Kamath, D. Jones, H. Tagoh, C. Bonifer, D. A. Hildeman, and K. J. Huang Regulation of the Interleukin-7 Receptor {alpha} Promoter by the Ets Transcription Factors PU.1 and GA-binding Protein in Developing B Cells J. Biol. Chem., May 11, 2007; 282(19): 14194 - 14204. [Abstract] [Full Text] [PDF]

				A. G. Bert, B. V. Johnson, E. W. Baxter, and P. N. Cockerill A Modular Enhancer Is Differentially Regulated by GATA and NFAT Elements That Direct Different Tissue-Specific Patterns of Nucleosome Positioning and Inducible Chromatin Remodeling Mol. Cell. Biol., April 15, 2007; 27(8): 2870 - 2885. [Abstract] [Full Text] [PDF]

				A. Kim, C. M. Kiefer, and A. Dean Distinctive Signatures of Histone Methylation in Transcribed Coding and Noncoding Human {beta}-Globin Sequences Mol. Cell. Biol., February 15, 2007; 27(4): 1271 - 1279. [Abstract] [Full Text] [PDF]

				H. Krysinska, M. Hoogenkamp, R. Ingram, N. Wilson, H. Tagoh, P. Laslo, H. Singh, and C. Bonifer A Two-Step, PU.1-Dependent Mechanism for Developmentally Regulated Chromatin Remodeling and Transcription of the c-fms Gene Mol. Cell. Biol., February 1, 2007; 27(3): 878 - 887. [Abstract] [Full Text] [PDF]

				F. A. Myers, P. Lefevre, E. Mantouvalou, K. Bruce, C. Lacroix, C. Bonifer, A. W. Thorne, and C. Crane-Robinson Developmental activation of the lysozyme gene in chicken macrophage cells is linked to core histone acetylation at its enhancer elements Nucleic Acids Res., September 1, 2006; 34(14): 4025 - 4035. [Abstract] [Full Text] [PDF]