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J. Biol. Chem., Vol. 280, Issue 30, 27662-27669, July 29, 2005

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µ and Opioid Receptors Activate ERK/MAPK via Different Protein Kinase C Isoforms and Secondary Messengers in Astrocytes^*

Mariana M. Belcheva, Amy L. Clark, Paul D. Haas, Jannie S. Serna, Jason W. Hahn, Alexi Kiss, and Carmine J. Coscia

From the E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, Missouri 63104

Acute µ and opioids activate the ERK/MAPK phosphorylation cascade that represents an integral part of the signaling pathway of growth factors in astrocytes. By this cross-talk, opioids may impact neural development and plasticity among other basic neurobiological processes in vivo. The µ agonist, [D-ala²,mephe⁴,glyol⁵]enkephalin (DAMGO), induces a transient stimulation of ERK phosphorylation, whereas agonist, U69,593, engenders sustained ERK activation. Here we demonstrate that acute U69,593 and DAMGO stimulate ERK phosphorylation by utilization of different secondary messengers and protein kinase C (PKC) isoforms upstream of the growth factor pathway. Immortalized astrocytes transfected with either antisense calmodulin (CaM), a mutant µ opioid receptor that binds CaM poorly or a dominant negative mutant of PKC were used as a model system to study µ signaling. Evidence was gained to implicate CaM and PKC in DAMGO stimulation of ERK. DAMGO activation of PKC and/or ERK was insensitive to selective inhibitors of Ca²⁺ mobilization, but it was blocked upon phospholipase C inhibition. These results suggest a novel mechanism wherein, upon DAMGO binding, CaM is released from the µ receptor and activates phospholipase C. Subsequently, phospholipase C generates diacylglycerides that activate PKC. In contrast, U69,593 appears to act via phosphoinositide 3-kinase, PKC, and Ca²⁺ mobilization. These signaling components were implicated based on studies with specific inhibitors and a dominant negative mutant of PKC. Collectively, our findings on acute opioid effects suggest that differences in their mechanism of signaling may contribute to the distinct outcomes on ERK modulation induced by chronic µ and opioids.

Received for publication, March 8, 2005 , and in revised form, June 7, 2005.

^* This work was supported in part by National Institutes of Health Grant DA05412. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, St. Louis University School of Medicine, 1402 S. Grand Blvd., St. Louis, MO 63104. Tel.: 314-977-9254; Fax: 314-977-9205; E-mail: cosciacc{at}slu.edu.

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