HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 30, 28085-28094, July 29, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/30/28085    most recent M500265200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doherty, K. M. Articles by Brosh, R. M. Search for Related Content PubMed PubMed Citation Articles by Doherty, K. M. Articles by Brosh, R. M., Jr. Social Bookmarking                      What's this?

RECQ1 Helicase Interacts with Human Mismatch Repair Factors That Regulate Genetic Recombination^*

Kevin M. Doherty, Sudha Sharma, Laura A. Uzdilla, Teresa M. Wilson, Sheng Cui¶, Alessandro Vindigni¶, and Robert M. Brosh, Jr.||

From the Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, Department of Radiation Oncology, Radiation Oncology Research Laboratory, University of Maryland, Baltimore, Maryland 21201, and ^¶International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy

Understanding the molecular and cellular functions of RecQ helicases has attracted considerable interest since several human diseases characterized by premature aging and/or cancer have been genetically linked to mutations in genes of the RecQ family. Although a human disease has not yet been genetically linked to a mutation in RECQ1, the prominent roles of RecQ helicases in the maintenance of genome stability suggest that RECQ1 helicase is likely to be important in vivo.To acquire a better understanding of RECQ1 cellular and molecular functions, we have investigated its protein interactions. Using a co-immunoprecipitation approach, we have identified several DNA repair factors that are associated with RECQ1 in vivo. Direct physical interaction of these repair factors with RECQ1 was confirmed with purified recombinant proteins. Importantly, RECQ1 stimulates the incision activity of human exonuclease 1 and the mismatch repair recognition complex MSH2/6 stimulates RECQ1 helicase activity. These protein interactions suggest a role of RECQ1 in a pathway involving mismatch repair factors. Regulation of genetic recombination, a proposed role for RecQ helicases, is supported by the identified RECQ1 protein interactions and is discussed.

Received for publication, January 7, 2005 , and in revised form, May 6, 2005.

^* This work was supported in part by National Institutes of Health Grant CA095690 (to T. M. W.) and Human Frontier Science Program grant, Fondo gli investmenti della Ricera di Base grant of Ministero dell'Istruzione dell'Universita e della Ricerca, and Consiglio Nazionale delle Ricerche, (Rome) Grant 02.00648.ST97 (to A. V.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Fig. 1.

^|| To whom correspondence should be addressed: Laboratory of Molecular Gerontology, NIA, National Institutes of Health, 5600 Nathan Shock Dr., Baltimore, MD 21224. Tel.: 410-558-8578; Fax: 410-558-8157; E-mail: BroshR{at}grc.nia.nih.gov.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. V. Bugreev, R. M. Brosh Jr., and A. V. Mazin RECQ1 Possesses DNA Branch Migration Activity J. Biol. Chem., July 18, 2008; 283(29): 20231 - 20242. [Abstract] [Full Text] [PDF]

				M. El-Shemerly, D. Hess, A. K. Pyakurel, S. Moselhy, and S. Ferrari ATR-dependent pathways control hEXO1 stability in response to stalled forks Nucleic Acids Res., February 2, 2008; 36(2): 511 - 519. [Abstract] [Full Text] [PDF]

				R. M. Brosh Jr and V. A. Bohr Human premature aging, DNA repair and RecQ helicases Nucleic Acids Res., December 3, 2007; 35(22): 7527 - 7544. [Abstract] [Full Text] [PDF]

				N. Saydam, R. Kanagaraj, T. Dietschy, P. L. Garcia, J. Pena-Diaz, I. Shevelev, I. Stagljar, and P. Janscak Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors Nucleic Acids Res., September 27, 2007; 35(17): 5706 - 5716. [Abstract] [Full Text] [PDF]

				J. Hejna, S. Philip, J. Ott, C. Faulkner, and R. Moses The hSNM1 protein is a DNA 5'-exonuclease Nucleic Acids Res., September 25, 2007; 35(18): 6115 - 6123. [Abstract] [Full Text] [PDF]

				S. Sharma, D. J. Stumpo, A. S. Balajee, C. B. Bock, P. M. Lansdorp, R. M. Brosh Jr., and P. J. Blackshear RECQL, a Member of the RecQ Family of DNA Helicases, Suppresses Chromosomal Instability Mol. Cell. Biol., March 1, 2007; 27(5): 1784 - 1794. [Abstract] [Full Text] [PDF]

				D. Li, M. Frazier, D. B. Evans, K. R. Hess, C. H. Crane, L. Jiao, and J. L. Abbruzzese Single Nucleotide Polymorphisms of RecQ1, RAD54L, and ATM Genes Are Associated With Reduced Survival of Pancreatic Cancer J. Clin. Oncol., April 10, 2006; 24(11): 1720 - 1728. [Abstract] [Full Text] [PDF]

				D. Li, H. Liu, L. Jiao, D. Z. Chang, G. Beinart, R. A. Wolff, D. B. Evans, M. M. Hassan, and J. L. Abbruzzese Significant effect of homologous recombination DNA repair gene polymorphisms on pancreatic cancer survival. Cancer Res., March 15, 2006; 66(6): 3323 - 3330. [Abstract] [Full Text] [PDF]

				S. Sharma, J. A. Sommers, S. Choudhary, J. K. Faulkner, S. Cui, L. Andreoli, L. Muzzolini, A. Vindigni, and R. M. Brosh Jr. Biochemical Analysis of the DNA Unwinding and Strand Annealing Activities Catalyzed by Human RECQ1 J. Biol. Chem., July 29, 2005; 280(30): 28072 - 28084. [Abstract] [Full Text] [PDF]