HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 31, 28281-28289, August 5, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/31/28281    most recent M503035200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ertel, F. Articles by Schleiff, E. Search for Related Content PubMed PubMed Citation Articles by Ertel, F. Articles by Schleiff, E. Social Bookmarking                      What's this?

The Evolutionarily Related -Barrel Polypeptide Transporters from Pisum sativum and Nostoc PCC7120 Contain Two Distinct Functional Domains^*

Franziska Ertel, Oliver Mirus, Rolf Bredemeier, Suncana Moslavac, Thomas Becker, and Enrico Schleiff

From the Department of Biology I, Ludwig Maximilians University, Menzinger Strasse 67, 80638 München, Germany

Several -barrel-type channels are involved in the translocation or assembly of outer membrane proteins of bacteria or endosymbiotically derived organelles. Here we analyzed the functional units of the -barrel polypeptide transporter Toc75 (translocon in outer envelope of chloroplasts) of the outer envelope of chloroplasts and of a protein, alr2269, from Nostoc PCC7120 with homology to Toc75, both proteins having a similar domain organization. We demonstrated that the N-terminal region functions as a recognition and complex assembly unit, whereas the C terminus forms the -barrel-type pore. The pore region is, in turn, modulated by the N terminus of the proteins. The protein from Nostoc PCC7120, which shares a common ancestor with Toc75, is able to recognize precursor proteins destined for chloroplasts. In contrast, the recognition of peripheral translocon subunits by Toc75 is a novel feature acquired through evolution.

Received for publication, March 18, 2005 , and in revised form, June 8, 2005.

^* This work was supported by grants from the Deutsche Forschungsgemeinschaft, Fonds der Chemischen Industrie, and the Volkswagenstiftung (to E. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a supplemental table and a figure.

To whom correspondence should be addressed: Dept. of Biology I, Ludwig Maximilians University, Menzinger Str. 67, 80638 München, Germany. Tel.: 49-89-17861-182; Fax: 49-89-17861-185; E-mail: schleiff{at}lrz.uni-muenchen.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Nesper, A. Brosig, P. Ringler, G. J. Patel, S. A. Muller, J. H. Kleinschmidt, W. Boos, K. Diederichs, and W. Welte Omp85Tt from Thermus thermophilus HB27: an Ancestral Type of the Omp85 Protein Family J. Bacteriol., July 1, 2008; 190(13): 4568 - 4575. [Abstract] [Full Text] [PDF]

				M. Oreb, A. Hofle, O. Mirus, and E. Schleiff Phosphorylation regulates the assembly of chloroplast import machinery J. Exp. Bot., June 1, 2008; 59(9): 2309 - 2316. [Abstract] [Full Text] [PDF]

				M. Kalanon and G. I. McFadden The Chloroplast Protein Translocation Complexes of Chlamydomonas reinhardtii: A Bioinformatic Comparison of Toc and Tic Components in Plants, Green Algae and Red Algae Genetics, May 1, 2008; 179(1): 95 - 112. [Abstract] [Full Text] [PDF]

				P. Vuong, D. Bennion, J. Mantei, D. Frost, and R. Misra Analysis of YfgL and YaeT Interactions through Bioinformatics, Mutagenesis, and Biochemistry J. Bacteriol., March 1, 2008; 190(5): 1507 - 1517. [Abstract] [Full Text] [PDF]

				S. Moslavac, K. Nicolaisen, O. Mirus, F. Al Dehni, R. Pernil, E. Flores, I. Maldener, and E. Schleiff A TolC-Like Protein Is Required for Heterocyst Development in Anabaena sp. Strain PCC 7120 J. Bacteriol., November 1, 2007; 189(21): 7887 - 7895. [Abstract] [Full Text] [PDF]

				S. Kim, J. C. Malinverni, P. Sliz, T. J. Silhavy, S. C. Harrison, and D. Kahne Structure and Function of an Essential Component of the Outer Membrane Protein Assembly Machine Science, August 17, 2007; 317(5840): 961 - 964. [Abstract] [Full Text] [PDF]

				R. Bredemeier, T. Schlegel, F. Ertel, A. Vojta, L. Borissenko, M. T. Bohnsack, M. Groll, A. von Haeseler, and E. Schleiff Functional and Phylogenetic Properties of the Pore-forming beta-Barrel Transporters of the Omp85 Family J. Biol. Chem., January 19, 2007; 282(3): 1882 - 1890. [Abstract] [Full Text] [PDF]

				S. J. Habib, T. Waizenegger, A. Niewienda, S. A. Paschen, W. Neupert, and D. Rapaport The N-terminal domain of Tob55 has a receptor-like function in the biogenesis of mitochondrial {beta}-barrel proteins J. Cell Biol., January 1, 2007; 176(1): 77 - 88. [Abstract] [Full Text] [PDF]

				J. F. Stegmeier and C. Andersen Characterization of Pores Formed by YaeT (Omp85) from Escherichia coli J. Biochem., August 1, 2006; 140(2): 275 - 283. [Abstract] [Full Text] [PDF]

				A. C. Meli, H. Hodak, B. Clantin, C. Locht, G. Molle, F. Jacob-Dubuisson, and N. Saint Channel Properties of TpsB Transporter FhaC Point to Two Functional Domains with a C-terminal Protein-conducting Pore J. Biol. Chem., January 6, 2006; 281(1): 158 - 166. [Abstract] [Full Text] [PDF]