HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 32, 28852-28857, August 12, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/32/28852    most recent M413750200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bernard, R. Articles by Denizot, F. Search for Related Content PubMed PubMed Citation Articles by Bernard, R. Articles by Denizot, F. Social Bookmarking                      What's this?

BcrC from Bacillus subtilis Acts as an Undecaprenyl Pyrophosphate Phosphatase in Bacitracin Resistance^*

Remi Bernard, Meriem El Ghachi¶, Dominique Mengin-Lecreulx¶, Marc Chippaux, and François Denizot||

From the Laboratoire de Chimie Bactérienne, Institut de Biologie Structurale et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20 and ^¶Laboratoire des Enveloppes Bactériennes et Antibiotiques, Institut de Biochimie et Biophysique Moléculaire et Cellulaire, UMR 8619 CNRS, Université Paris-Sud, Btiment 430, 91405 Orsay Cedex, France

Overexpression of the BcrC_Bs protein, formerly called YwoA, in Escherichia coli or in Bacillus subtilis allows these bacteria to stand higher concentrations of bacitracin. It was suggested that BcrC_Bs was a membrane-spanning domain of an ATP binding cassette (ABC) transporter involved in bacitracin resistance. However, we hypothesized that this protein has an undecaprenyl pyrophosphate (UPP) phosphatase activity able to compete with bacitracin for UPP. We found that overexpression of a recombinant His₆-BcrC_Bs protein in E. coli (i) increased the resistance of the cells to bacitracin and (ii) increased UPP phosphatase activity in membrane preparations by 600-fold. We solubilized and prepared an electrophoretically pure protein exhibiting a strong UPP phosphatase activity. BcrC_Bs, which belongs to the type 2 phosphatidic acid phosphatase (PAP2) phosphatase superfamily (PF01569), differs totally from the already known BacA UPP phosphatase from E. coli, a member of the PF02673 family of the Protein family (Pfam) database. Thus, BcrC_Bs and its orthologs form a new class of proteins within the PAP2 phosphatase superfamily, and likely all of them share a UPP phosphatase activity.

Received for publication, December 7, 2004 , and in revised form, May 31, 2005.

^* This work was supported by grants from the Centre National de la Recherche Scientifique (to D. M.-L. and F. D.) and by grants from the European Community (Grant FP6, LSHM-CT-2003-503335, "COBRA" project) (to D. M.-L.) and from Hoechst Marion Roussel (Grant FRHMR2/9932) (to F. D.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipients of a scholarship from the Ministère de l'Education Nationale, de la Recherche et de la Technologie.

^|| To whom correspondence should be addressed. Tel.: 33-4-91164387; Fax: 33-4-91718914; E-mail: denizot{at}ibsm.cnrs-mrs.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C.-L. Tseng and G.-C. Shaw Genetic Evidence for the Actin Homolog Gene mreBH and the Bacitracin Resistance Gene bcrC as Targets of the Alternative Sigma Factor SigI of Bacillus subtilis J. Bacteriol., March 1, 2008; 190(5): 1561 - 1567. [Abstract] [Full Text] [PDF]

				R. Bernard, A. Guiseppi, M. Chippaux, M. Foglino, and F. Denizot Resistance to Bacitracin in Bacillus subtilis: Unexpected Requirement of the BceAB ABC Transporter in the Control of Expression of Its Own Structural Genes J. Bacteriol., December 1, 2007; 189(23): 8636 - 8642. [Abstract] [Full Text] [PDF]

				A. J. Jervis, P. D. Thackray, C. W. Houston, M. J. Horsburgh, and A. Moir SigM-Responsive Genes of Bacillus subtilis and Their Promoters J. Bacteriol., June 15, 2007; 189(12): 4534 - 4538. [Abstract] [Full Text] [PDF]

				T. Wecke, B. Veith, A. Ehrenreich, and T. Mascher Cell Envelope Stress Response in Bacillus licheniformis: Integrating Comparative Genomics, Transcriptional Profiling, and Regulon Mining To Decipher a Complex Regulatory Network J. Bacteriol., November 1, 2006; 188(21): 7500 - 7511. [Abstract] [Full Text] [PDF]