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J. Biol. Chem., Vol. 280, Issue 32, 29334-29339, August 12, 2005

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Processing of Viral DNA Ends Channels the HIV-1 Integration Reaction to Concerted Integration^*

Min Li and Robert Craigie

From the Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892

Retroviral DNA made by reverse transcription is blunt-ended, and the viral integrase protein must remove two nucleotides from each 3' end prior to integration into chromosomal DNA. Under most reaction conditions for integration in vitro, the majority of the reaction products are "half-site" products that result from integration of only one viral DNA end into one strand of the target DNA. Preprocessed DNA substrates are more efficient substrates for half-site reactions than are blunt-ended substrates, which require the removal of two nucleotides prior to integration. In contrast, we find that blunt-ended DNA is a better substrate for the biologically relevant reaction of concerted integration of pairs of viral DNA ends. The reaction pathway is channeled to concerted integration, and half-site integration products are reduced with blunt-ended DNA substrate that must first be processed by integrase. In addition, the terminal nucleotide requirements for concerted integration are more stringent than for the half-site reaction. Longer DNA is more efficient for the concerted reaction than is shorter DNA that is capable of efficient half-site integration. This suggests that nonspecific interactions of integrase with viral DNA distant from the termini contribute to the assembly of a complex that is competent for concerted integration. Finally, differential effects of mutation of a residue in the C-terminal domain of integrase on concerted versus half-site integration implicate protein-protein interactions involving this domain as important for concerted integration.

Received for publication, May 17, 2005 , and in revised form, June 14, 2005.

^* This work was supported in part by the National Institutes of Health AIDS Targeted Antiviral Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a supplemental figure and a supplemental table.

To whom correspondence should be addressed: Laboratory of Molecular Biology, NIDDK, Bldg. 5, Rm. 301, 5 Center Dr. MSC 0560, National Institutes of Health, Bethesda, MD 20892. Tel.: 301-496-4081; Fax: 301-496-0201; E-mail: bobc{at}helix.nih.gov.

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