HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 36, 31397-31404, September 9, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/36/31397    most recent M503277200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnson, A. Articles by Tzagoloff, A. Search for Related Content PubMed PubMed Citation Articles by Johnson, A. Articles by Tzagoloff, A. Social Bookmarking                      What's this?

COQ9, a New Gene Required for the Biosynthesis of Coenzyme Q in Saccharomyces cerevisiae^*

Alisha Johnson, Peter Gin, Beth N. Marbois, Edward J. Hsieh, Mian Wu¶, Mario H. Barros, Catherine F. Clarke, and Alexander Tzagoloff||

From the Department of Biological Sciences, Columbia University, New York, New York 10027 and the Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095

Currently, eight genes are known to be involved in coenzyme Q₆ biosynthesis in Saccharomyces cerevisiae. Here, we report a new gene designated COQ9 that is also required for the biosynthesis of this lipoid quinone. The respiratory-deficient pet mutant C92 was found to be deficient in coenzyme Q and to have low mitochondrial NADH-cytochrome c reductase activity, which could be restored by addition of coenzyme Q₂. The mutant was used to clone COQ9, corresponding to reading frame YLR201c on chromosome XII. The respiratory defect of C92 is complemented by COQ9 and suppressed by COQ8/ ABC1. The latter gene has been shown to be required for coenzyme Q biosynthesis in yeast and bacteria. Suppression by COQ8/ABC1 of C92, but not other coq9 mutants tested, has been related to an increase in the mitochondrial concentration of several enzymes of the pathway. Coq9p may either catalyze a reaction in the coenzyme Q biosynthetic pathway or have a regulatory role similar to that proposed for Coq8p.

Received for publication, March 24, 2005 , and in revised form, July 13, 2005.

^* This work was supported by National Institutes of Health Research Grants HL2274 (to A. T.) and GM45952 (to C. F. C.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ Present address: Dept. of Molecular and Cell Biology, Key Laboratory of Structural Biology, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.

^|| To whom correspondence should be addressed: Dept. of Biological Sciences, Columbia University, New York, NY 10027. Tel.: 212-854-2920; Fax: 212-856-8246; E-mail: spud{at}cubpet2.bio.columbia.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Gulmezian, H. Zhang, G. T. Javor, and C. F. Clarke Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis. J. Bacteriol., September 1, 2006; 188(17): 6435 - 6439. [Abstract] [Full Text] [PDF]

				U. C. Tran, B. Marbois, P. Gin, M. Gulmezian, T. Jonassen, and C. F. Clarke Complementation of Saccharomyces cerevisiae coq7 Mutants by Mitochondrial Targeting of the Escherichia coli UbiF Polypeptide: TWO FUNCTIONS OF YEAST COQ7 POLYPEPTIDE IN COENZYME Q BIOSYNTHESIS J. Biol. Chem., June 16, 2006; 281(24): 16401 - 16409. [Abstract] [Full Text] [PDF]

				M. H. Barros, A. Johnson, P. Gin, B. N. Marbois, C. F. Clarke, and A. Tzagoloff The Saccharomyces cerevisiae COQ10 Gene Encodes a START Domain Protein Required for Function of Coenzyme Q in Respiration J. Biol. Chem., December 30, 2005; 280(52): 42627 - 42635. [Abstract] [Full Text] [PDF]