HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 4, 2596-2605, January 28, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/4/2596    most recent M408938200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mostecki, J. Articles by Rothman, P. B. Search for Related Content PubMed PubMed Citation Articles by Mostecki, J. Articles by Rothman, P. B. Social Bookmarking                      What's this?

Early Growth Response-1 Regulates Lipopolysaccharide-induced Suppressor of Cytokine Signaling-1 Transcription^*

Justin Mostecki, Brian M. Showalter, and Paul B. Rothman¶

From the Department of Microbiology and Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032

Suppressor of cytokine signaling (SOCS)-1 is a critical regulator of lipopolysaccharide (LPS) tolerance and LPS-induced cytokine production. The mechanisms regulating the transcription of SOCS-1 in response to LPS are not entirely understood. Functional analysis of the SOCS-1 promoter demonstrates that early growth response-1 (Egr-1) is an important transcriptional regulator of SOCS-1. Two Egr-1 binding sites are present within the SOCS-1 promoter as shown by EMSA and supershift analysis. Further, mutation of the Egr-1 binding sites significantly reduces both the basal and LPS-induced transcriptional activity of the promoter. Chromatin immunoprecipitation experiments confirm LPS-induced binding of Egr-1 to the SOCS-1 promoter in vivo. Additionally, Egr-1^–/– macrophages show reduced levels of LPS-induced SOCS-1 expression in comparison with macrophages derived from Egr-1^+/+ littermate controls. These results demonstrate an important role for Egr-1 in regulating both the basal and LPS-induced activity of the SOCS-1 promoter.

Received for publication, August 4, 2004 , and in revised form, October 27, 2004.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Medicine, Division of Pulmonary, Allergy and Critical Care, P&S Bldg., Rm. 8-425, Columbia University, 630 W. 168th St., New York, NY 10032. Tel.: 212-305-6982; Fax: 212-305-1870; E-mail: pbr3{at}columbia.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Schedel, L. A. Pinto, B. Schaub, P. Rosenstiel, D. Cherkasov, L. Cameron, N. Klopp, T. Illig, C. Vogelberg, S. K. Weiland, et al. IRF-1 Gene Variations Influence IgE Regulation and Atopy Am. J. Respir. Crit. Care Med., March 15, 2008; 177(6): 613 - 621. [Abstract] [Full Text] [PDF]

				S.-J. Chen, H. Ning, W. Ishida, S. Sodin-Semrl, S. Takagawa, Y. Mori, and J. Varga The Early-Immediate Gene EGR-1 Is Induced by Transforming Growth Factor-beta and Mediates Stimulation of Collagen Gene Expression J. Biol. Chem., July 28, 2006; 281(30): 21183 - 21197. [Abstract] [Full Text] [PDF]

				H. Qin, C. A. Wilson, S. J. Lee, and E. N. Benveniste IFN-{beta}-induced SOCS-1 negatively regulates CD40 gene expression in macrophages and microglia FASEB J, May 1, 2006; 20(7): 985 - 987. [Abstract] [Full Text] [PDF]

				A. Kimura, T. Naka, T. Muta, O. Takeuchi, S. Akira, I. Kawase, and T. Kishimoto Suppressor of cytokine signaling-1 selectively inhibits LPS-induced IL-6 production by regulating JAK-STAT PNAS, November 22, 2005; 102(47): 17089 - 17094. [Abstract] [Full Text] [PDF]