HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 4, 2708-2713, January 28, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/4/2708    most recent M411786200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Renault, M.-A. Articles by Gadeau, A.-P. Search for Related Content PubMed PubMed Citation Articles by Renault, M.-A. Articles by Gadeau, A.-P. Social Bookmarking                      What's this?

UTP Induces Osteopontin Expression through a Coordinate Action of NFB, Activator Protein-1, and Upstream Stimulatory Factor in Arterial Smooth Muscle Cells^*

Marie-Ange Renault, Sandra Jalvy, Mylène Potier, Isabelle Belloc, Elisabeth Genot||, Lodewijk V. Dekker**, Claude Desgranges, and Alain-Pierre Gadeau¶

From the INSERM, U441 Athérosclérose, Bordeaux 33600, France, Université Victor Segalen Bordeaux 2, Bordeaux 33076, France, ^||Université Bordeaux 1, Institut Européen de Chimie et de Biologie, Bordeaux 33600, France, and ^**Ionix Pharmaceuticals Ltd., Cambridge CB4 0PA, United Kingdom

Osteopontin (OPN) is an important chemokinetic agent for several cell types. Our earlier studies have shown that its expression is essential for uridine triphosphate (UTP)-mediated migration of vascular smooth muscle cells. We demonstrated previously that the activation of an AP-1 binding site located 76 bp upstream of the transcription start in the rat OPN promoter is involved in the induction of OPN expression. In this work, using a luciferase promoter deletion assay, we identified a new region of the rat OPN promoter (-1837 to -1757) that is responsive to UTP. This region contains an NFB site located at -1800 and an Ebox located at -1768. Supershift electrophoretic mobility shift assay and chromatin immunoprecipitation assays identified NFB and USF-1/USF-2 as the DNA binding proteins induced by UTP, respectively, for these two sites. Using dominant negative mutants of IB kinase and USF transcription factors, we confirmed that NFB and USF-1/USF-2 are involved in the UTP-mediated expression of OPN. Using a pharmacological approach, we demonstrated that USF proteins are regulated by the extracellular signal-regulated kinase (ERK)1/2 pathway, just as the earlier discovered AP-1 complex, whereas NFB is up-regulated through PKC signals. Finally, our work suggests that the UTP-stimulated OPN expression involves a coordinate regulation of PKC-NFB, ERK1/2-USF, and ERK1/2/NAD(P)H oxidase AP-1 signaling pathways.

Received for publication, October 18, 2004

^* This study was supported by grants from the Institut National de la Santé et de la Recherche Médicale, University of Bordeaux II, the Fondation de France (Programme Santé), and by fellowships from the Société Française d'Hypertension Artérielle and the Fondation pour la Recherche Médicale (to M.-A. R.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: INSERM U441, Ave. du Haut Lévêque, 33600 Pessac, France. Tel.: 33-5-57-89-19-79; Fax: 33-5-56-36-89-79; E-mail: alain.gadeau{at}bordeaux.inserm.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T.-C. Lu, Z. Wang, X. Feng, P. Chuang, W. Fang, Y. Chen, S. Neves, A. Maayan, H. Xiong, Y. Liu, et al. Retinoic Acid Utilizes CREB and USF1 in a Transcriptional Feed-Forward Loop in Order To Stimulate MKP1 Expression in Human Immunodeficiency Virus-Infected Podocytes Mol. Cell. Biol., September 15, 2008; 28(18): 5785 - 5794. [Abstract] [Full Text] [PDF]

				K. Kristiansson, E. Ilveskoski, T. Lehtimaki, L. Peltonen, M. Perola, and P. J. Karhunen Association Analysis of Allelic Variants of USF1 in Coronary Atherosclerosis Arterioscler. Thromb. Vasc. Biol., May 1, 2008; 28(5): 983 - 989. [Abstract] [Full Text] [PDF]

				J. Irita, T. Okura, M. Kurata, K.-i. Miyoshi, T. Fukuoka, and J. Higaki Osteopontin in Rat Renal Fibroblasts: Functional Properties and Transcriptional Regulation by Aldosterone Hypertension, February 1, 2008; 51(2): 507 - 513. [Abstract] [Full Text] [PDF]

				S. Jalvy, M.-A. Renault, L. L. S. Leen, I. Belloc, J. Bonnet, A.-P. Gadeau, and C. Desgranges Autocrine expression of osteopontin contributes to PDGF-mediated arterial smooth muscle cell migration Cardiovasc Res, September 1, 2007; 75(4): 738 - 747. [Abstract] [Full Text] [PDF]

				S. Jalvy, M.-A. Renault, L. Lam Shang Leen, I. Belloc, A. Reynaud, A.-P. Gadeau, and C. Desgranges CREB Mediates UTP-Directed Arterial Smooth Muscle Cell Migration and Expression of the Chemotactic Protein Osteopontin via Its Interaction with Activator Protein-1 Sites Circ. Res., May 11, 2007; 100(9): 1292 - 1299. [Abstract] [Full Text] [PDF]