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Originally published In Press as doi:10.1074/jbc.M411555200 on November 23, 2004

J. Biol. Chem., Vol. 280, Issue 4, 2954-2961, January 28, 2005
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Serum Amyloid A Is a Ligand for Scavenger Receptor Class B Type I and Inhibits High Density Lipoprotein Binding and Selective Lipid Uptake*

Lei Cai, Maria C. de Beer, Frederick C. de Beer, and Deneys R. van der Westhuyzen{ddagger}

From the Department of Internal Medicine, Graduate Center for Nutritional Sciences, University of Kentucky Medical Center, Lexington, Kentucky 40536 and the Department of Veterans Affairs Medical Center, Lexington, Kentucky 40511

Serum amyloid A is an acute phase protein that is carried in the plasma largely as an apolipoprotein of high density lipoprotein (HDL). In this study we investigated whether SAA is a ligand for the HDL receptor, scavenger receptor class B type I (SR-BI), and how SAA may influence SR-BI-mediated HDL binding and selective cholesteryl ester uptake. Studies using Chinese hamster ovary cells expressing SR-BI showed that 125I-labeled SAA, both in lipid-free form and in reconstituted HDL particles, functions as a high affinity ligand for SR-BI. SAA also bound with high affinity to the hepatocyte cell line, HepG2. Alexa-labeled SAA was shown by fluorescence confocal microscopy to be internalized by cells in a SR-BI-dependent manner. To assess how SAA association with HDL influences HDL interaction with SR-BI, SAA-containing HDL was isolated from mice overexpressing SAA through adenoviral gene transfer. SAA presence on HDL had little effect on HDL binding to SR-BI but decreased (30–50%) selective cholesteryl ester uptake. Lipid-free SAA, unlike lipid-free apoA-I, was an effective inhibitor of both SR-BI-dependent binding and selective cholesteryl ester uptake of HDL. We have concluded that SR-BI plays a key role in SAA metabolism through its ability to interact with and internalize SAA and, further, that SAA influences HDL cholesterol metabolism through its inhibitory effects on SR-BI-mediated selective lipid uptake.


Received for publication, October 12, 2004 , and in revised form, November 23, 2004.

* This work was supported by National Institutes of Health Grants HL-63763 and HL-65730 (to D. R. v. d. W.) and AG-17237 (to F. C. d. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

{ddagger} To whom correspondence should be addressed: Dept. of Internal Medicine and Graduate Center for Nutritional Sciences, Wethington Health Science Bldg. 541, 900 S. Limestone St., Lexington, KY 40536. Tel.: 859-323-4933 (ext. 81397); Fax: 859-323-5707; E-mail: dvwest1{at}uky.edu.


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