HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 41, 34558-34568, October 14, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/41/34558    most recent M503465200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barth, S. Articles by Michaeli, S. Search for Related Content PubMed PubMed Citation Articles by Barth, S. Articles by Michaeli, S. Social Bookmarking                      What's this?

Elucidating the Role of H/ACA-like RNAs in trans-Splicing and rRNA Processing via RNA Interference Silencing of the Trypanosoma brucei CBF5 Pseudouridine Synthase^*

From the Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel

Most pseudouridinylation in eukaryotic rRNA and small nuclear RNAs is guided by H/ACA small nucleolar RNAs. In this study, the Trypanosoma brucei pseudouridine synthase, Cbf5p, a snoRNP protein, was identified and silenced by RNAi. Depletion of this protein destabilized all small nucleolar RNAs of the H/ACA-like family. Following silencing, defects in rRNA processing, such as accumulation of precursors and inhibition of cleavages to generate the mature rRNA, were observed. snR30, an H/ACA RNA involved in rRNA maturation, was identified based on prototypical conserved domains characteristic of this RNA in other eukaryotes. The silencing of CBF5 also eliminated the spliced leader-associated (SLA1) RNA that directs pseudouridylation on the spliced leader RNA (SL RNA), which is the substrate for the trans-splicing reaction. Surprisingly, the depletion of Cbf5p not only eliminated the pseudouridine on the SL RNA but also abolished capping at the fourth cap-4 nucleotide. As a result of defects in the SL RNA and decreased modification on the U small nuclear RNA, trans-splicing was inhibited at the first step of the reaction, providing evidence for the essential role of H/ACA RNAs and the modifications they guide on trans-splicing.

Received for publication, March 30, 2005 , and in revised form, August 8, 2005.

^* This work was supported by a grant from the Israel Science Foundation, (841/04), a grant from the United States-Israel Binational Science Foundation, and by an International Research Scholars grant from the Howard Hughes Foundation (to S. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1 and S2.

¹ Both authors contributed equally to this work.

² To whom correspondence should be addressed: Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. Tel.: 972-3-5318068; Fax: 972-3-5351824; E-mail: michaes{at}mail.biu.ac.il.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. R. Zamudio, B. Mittra, A. Chattopadhyay, J. A. Wohlschlegel, N. R. Sturm, and D. A. Campbell Trypanosoma brucei Spliced Leader RNA Maturation by the Cap 1 2'-O-Ribose Methyltransferase and SLA1 H/ACA snoRNA Pseudouridine Synthase Complex Mol. Cell. Biol., March 1, 2009; 29(5): 1202 - 1211. [Abstract] [Full Text] [PDF]

				A. Hury, H. Goldshmidt, I. D. Tkacz, and S. Michaeli Trypanosome Spliced-Leader-Associated RNA (SLA1) Localization and Implications for Spliced-Leader RNA Biogenesis Eukaryot. Cell, January 1, 2009; 8(1): 56 - 68. [Abstract] [Full Text] [PDF]

				H. Goldshmidt, L. Sheiner, P. Butikofer, I. Roditi, S. Uliel, M. Gunzel, M. Engstler, and S. Michaeli Role of Protein Translocation Pathways across the Endoplasmic Reticulum in Trypanosoma brucei J. Biol. Chem., November 14, 2008; 283(46): 32085 - 32098. [Abstract] [Full Text] [PDF]

				S. Barth, B. Shalem, A. Hury, I. D. Tkacz, X.-h. Liang, S. Uliel, I. Myslyuk, T. Doniger, M. Salmon-Divon, R. Unger, et al. Elucidating the Role of C/D snoRNA in rRNA Processing and Modification in Trypanosoma brucei Eukaryot. Cell, January 1, 2008; 7(1): 86 - 101. [Abstract] [Full Text] [PDF]

				X.-h. Liang, A. Hury, E. Hoze, S. Uliel, I. Myslyuk, A. Apatoff, R. Unger, and S. Michaeli Genome-Wide Analysis of C/D and H/ACA-Like Small Nucleolar RNAs in Leishmania major Indicates Conservation among Trypanosomatids in the Repertoire and in Their rRNA Targets Eukaryot. Cell, March 1, 2007; 6(3): 361 - 377. [Abstract] [Full Text] [PDF]