|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 280, Issue 41, 34859-34869, October 14, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Angiomotin Regulates Endothelial Cell-Cell Junctions and Cell Motility*
From the Department of Oncology and Pathology, Cancer Centrum Karolinska, Karolinska Institutet, R8:03 Karolinska University Hospital, 171 76 Stockholm, Sweden
We have previously identified angiomotin by its ability to bind to and mediate the anti-angiogenic properties of angiostatin. In vivo and in vitro data indicate an essential role of angiomotin in endothelial cell motility. Here we show that angiostatin binds angiomotin on the cell surface and provide evidence for a transmembrane model for the topology of both p80 and p130 angiomotin isoforms. Immunofluorescence analysis shows that angiomotin co-localized with ZO-1 in cell-cell contacts in endothelial cells in vitro and in angiogenic blood vessels of the postnatal mouse retina in vivo. Transfection of p80 as well as p130 angiomotin in Chinese hamster ovary cells resulted in junctional localization of both isoforms. Furthermore, p130 angiomotin could recruit ZO-1 to actin stress fibers. The p130 but not p80 isoform could be coprecipitated with MAGI-1b, a component of endothelial tight junctions. Paracellular permeability, as measured by diffusion of fluorescein isothiocyanate-dextran, was reduced by p80 and p130 angiomotin expression with 70 and 88%, respectively, compared with control. Angiostatin did not have any effect on cell permeability but inhibited the migration of angiomotin-expressing cells in the Boyden chamber assay. We conclude that angiomotin, in addition to controlling cell motility, may play a role in the assembly of endothelial cell-cell junctions.
Received for publication, April 11, 2005 , and in revised form, June 29, 2005.
* This work was supported in part by grants from the Swedish Cancer Society, the Swedish Research Council, Cancerföreningen i Stockholm, and Karolinska Institutet. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1-S3.
1 Supported by a grant from the Wenner-Gren Foundation.
2 To whom correspondence should be addressed. Tel.: 46-8-517-79317; Fax: 46-8-339-031; E-mail: Lars.Holmgren{at}cck.ki.se.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Almog, L. Ma, R. Raychowdhury, C. Schwager, R. Erber, S. Short, L. Hlatky, P. Vajkoczy, P. E. Huber, J. Folkman, et al. Transcriptional Switch of Dormant Tumors to Fast-Growing Angiogenic Phenotype Cancer Res., February 1, 2009; 69(3): 836 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ernkvist, N. L. Persson, S. Audebert, P. Lecine, I. Sinha, M. Liu, M. Schlueter, A. Horowitz, K. Aase, T. Weide, et al. The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells Blood, January 1, 2009; 113(1): 244 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Horowitz and M. Simons Branching Morphogenesis Circ. Res., October 10, 2008; 103(8): 784 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Duning, E.-M. Schurek, M. Schluter, M. Bayer, H.-C. Reinhardt, A. Schwab, L. Schaefer, T. Benzing, B. Schermer, M. A. Saleem, et al. KIBRA Modulates Directional Migration of Podocytes J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1891 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Garnaas, K. L. Moodie, M.-l. Liu, G. V. Samant, K. Li, R. Marx, J. M. Baraban, A. Horowitz, and R. Ramchandran Syx, a RhoA Guanine Exchange Factor, Is Essential for Angiogenesis In Vivo Circ. Res., September 26, 2008; 103(7): 710 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Levchenko, N. Veitonmaki, A. Lundkvist, H. Gerhardt, Y. Ming, K. Berggren, A. Kvanta, R. Carlsson, and L. Holmgren Therapeutic antibodies targeting angiomotin inhibit angiogenesis in vivo FASEB J, March 1, 2008; 22(3): 880 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Perri, B. Annabi, and J. Galipeau Angiostatin inhibits monocyte/macrophage migration via disruption of actin cytoskeleton FASEB J, December 1, 2007; 21(14): 3928 - 3936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Aase, M. Ernkvist, L. Ebarasi, L. Jakobsson, A. Majumdar, C. Yi, O. Birot, Y. Ming, A. Kvanta, D. Edholm, et al. Angiomotin regulates endothelial cell migration during embryonic angiogenesis Genes & Dev., August 15, 2007; 21(16): 2055 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sugihara-Mizuno, M. Adachi, Y. Kobayashi, Y. Hamazaki, M. Nishimura, T. Imai, M. Furuse, and S. Tsukita Molecular characterization of angiomotin/JEAP family proteins: interaction with MUPP1/Patj and their endogenous properties Genes Cells, April 1, 2007; 12(4): 473 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Huang, F.-I Lu, S. Jia, S. Meng, Y. Cao, Y. Wang, W. Ma, K. Yin, Z. Wen, J. Peng, et al. Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation Development, March 1, 2007; 134(5): 979 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cannavo, B. Gerrits, G. Marra, R. Schlapbach, and J. Jiricny Characterization of the Interactome of the Human MutL Homologues MLH1, PMS1, and PMS2 J. Biol. Chem., February 2, 2007; 282(5): 2976 - 2986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Holmgren, E. Ambrosino, O. Birot, C. Tullus, N. Veitonmaki, T. Levchenko, L.-M. Carlson, P. Musiani, M. Iezzi, C. Curcio, et al. A DNA vaccine targeting angiomotin inhibits angiogenesis and suppresses tumor growth PNAS, June 13, 2006; 103(24): 9208 - 9213. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |