|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
J. Biol. Chem., Vol. 280, Issue 41, 35018-35027, October 14, 2005
Transcription of the NR1 Subunit of the N-Methyl-D-aspartate Receptor Is Down-regulated by Excitotoxic Stimulation and Cerebral Ischemia * 1 2 3![]() ![]() ![]() 4
From the
The N-methyl-D-aspartate (NMDA) type of glutamate receptor (NMDAR) plays central roles in normal and pathological neuronal functioning. We have examined the regulation of the NR1 subunit of the NMDAR in response to excessive activation of this receptor in in vitro and in vivo models of excitotoxicity. NR1 protein expression in cultured cortical neurons was specifically reduced by stimulation with 100 µM NMDA or glutamate. NMDA decreased NR1 protein amounts by 71% after 8 h. Low NMDA concentrations (
Received for publication, April 15, 2005 , and in revised form, July 22, 2005. * This work was supported by Grants SAF2003-02327 (to M. D.-G.), SAF2001-1624 (to A. R.-P.) and PM99-0115 (to J. R.) from the "Dirección General de Investigación Científica y Técnica" (Ministerio de Ciencia y Tecnología). 1 Recipient of a predoctoral fellowship from the Comunidad de Madrid. 2 Recipient of a predoctoral fellowship from Ministerio de Ciencia y Tecnología. 3 Recipient of a predoctoral fellowship from Fundación Teófilo Hernando. Present address: Instituto de Investigaciones Biomédicas "Alberto Sols," CSIC-UAM, Arturo Duperier, 4, 28029-Madrid, Spain. 4 Researcher of the Ramón y Cajal Program. To whom correspondence should be addressed. Tel.: 34-91-5854448; Fax: 34-91-5854401; E-mail: mdiazguerra{at}iib.uam.es.
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Advertisement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||