HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 41, 35028-35037, October 14, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/41/35028    most recent M505852200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hébert, R. L. Articles by Breyer, M. D. Search for Related Content PubMed PubMed Citation Articles by Hébert, R. L. Articles by Breyer, M. D. Social Bookmarking                      What's this?

Characterization of a Rabbit Kidney Prostaglandin F₂ Receptor Exhibiting G_i-restricted Signaling That Inhibits Water Absorption in the Collecting Duct^*

Richard L. Hébert, Monica Carmosino, Osamu Saito, Guangrui Yang¶, Cynthia A. Jackson, Zhonghua Qi, Richard M. Breyer, Chandramohan Natarajan, Aaron N. Hata, Yahua Zhang, Youfei Guan¶, and Matthew D. Breyer1

From the Departments of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada, the ^¶Department of Physiology, Peking University Health Science Center, Beijing, China 100083, and the Division of Nephrology, Vanderbilt University Medical Center, Nashville, Tennessee 37232

PGF₂ is the most abundant prostaglandin detected in urine; however, its renal effects are poorly characterized. The present study cloned a PGF-prostanoid receptor (FP) from the rabbit kidney and determined the functional consequences of its activation. Nuclease protection assay showed that FP mRNA expression predominates in rabbit ovary and kidney. In situ hybridization revealed that renal FP expression predominates in the cortical collecting duct (CCD). Although FP receptor activation failed to increase intracellular Ca²⁺, it potently inhibited vasopressin-stimulated osmotic water permeability (L_p, 10^-7 cm/(atm·s)) in in vitro microperfused rabbit CCDs. Inhibition of L_p by the FP selective agonist latanoprost was additive to inhibition of vasopressin action by the EP selective agonist sulprostone. Inhibition of L_p by latanoprost was completely blocked by pertussis toxin, consistent with a G_i-coupled mechanism. Heterologous transfection of the rabbit FPr into HEK293 cells also showed that latanoprost inhibited cAMP generation via a pertussis toxin-sensitive mechanism but did not increase cell Ca²⁺. These studies demonstrate a functional FP receptor on the basolateral membrane of rabbit CCDs. In contrast to the Ca²⁺ signal transduced by other FP receptors, this renal FP receptor signals via a PT-sensitive mechanism that is not coupled to cell Ca²⁺.

Received for publication, May 31, 2005 , and in revised form, July 21, 2005.

^* This work was supported by National Institutes of Health Grant DK37097 (to M. D. B.). This work was also supported by the Kidney Foundation of Canada and by Canadian Institutes Health Research Grant MT-14103 (to R. L. H.), by Major National Basic Research Program of China Grants G2000056908, 30271521, and 303400810 (to Y. G.), and by National Institutes of Health Grant DK46205 (to R. M. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Div. of Nephrology and VAMC Vanderbilt University Nashville, TN 37232. Tel.: 615-327-4751 (ext. 5492); Fax: 615-343-4704; E-mail:: matthew.breyer{at}vanderbilt.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. M. Smyth, T. Grosser, M. Wang, Y. Yu, and G. A. FitzGerald Prostanoids in health and disease J. Lipid Res., April 1, 2009; 50(Supplement): S423 - S428. [Abstract] [Full Text] [PDF]

				M. Pi, P. Faber, G. Ekema, P. D. Jackson, A. Ting, N. Wang, M. Fontilla-Poole, R. W. Mays, K. R. Brunden, J. J. Harrington, et al. Identification of a Novel Extracellular Cation-sensing G-protein-coupled Receptor J. Biol. Chem., December 2, 2005; 280(48): 40201 - 40209. [Abstract] [Full Text] [PDF]