HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 43, 36150-36157, October 28, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/43/36150    most recent M507706200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, L. Articles by Brand, J. G. Search for Related Content PubMed PubMed Citation Articles by Huang, L. Articles by Brand, J. G. Social Bookmarking                      What's this?

Identification and Functional Characterization of a Voltage-gated Chloride Channel and Its Novel Splice Variant in Taste Bud Cells^*

Liquan Huang1, Jie Cao, Hong Wang, Lynn A. Vo, and Joseph G. Brand¶

From the Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104-3308 and the Veterans Affairs Medical Center and the ^¶Department of Biochemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Taste bud cells are epithelial cells with neuronal properties. Voltage-dependent ion channels have been physiologically described in these cells. Here, we report the molecular identification and functional characterization of a voltage-gated chloride channel (ClC-4) and its novel splice variant (ClC-4A) from taste bud cells. ClC-4A skipped an exon near its 5'-end, incurring the loss of 60 amino acids at the N terminus. In situ hybridization and immunohistochemistry localized these two channels' transcripts and proteins to a subset of taste bud cells. Electrophysiological recordings of the heterologously expressed channels in Xenopus oocytes showed that ClC-4 and ClC-4A have opposite sensitivity to pH and unique ion selectivity. The chloride channel blockers niflumic acid and 5-nitro-2-(3-phenylpropylamino)benzoic acid had a slight or no inhibitory effect on the conductance of ClC-4, but both blockers inhibited ClC-4A, suggesting that ClC-4A is a candidate channel for an acid-induced 5-nitro-2-(3-phenylpropylamino)benzoic acid-sensitive current. Furthermore, these two channels may play a role in bitter-, sweet-, and umami-mediated taste transmission by regulating transmitter uptake into synaptic vesicles.

Received for publication, July 15, 2005 , and in revised form, August 26, 2005.

^* This work was supported by National Institutes of Health Grant DC05154 (to L. H.), a grant from the United States Department of Veterans Affairs (to J. G. B.), and National Science Foundation Equipment Grant DBI-0216310. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) DQ186662.

¹ To whom correspondence should be addressed: Monell Chemical Senses Center, 3500 Market St., Philadelphia, PA 19104-3308. Tel.: 215-746-0145; Fax: 215-898-2084; E-mail: lhuang{at}monell.org.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Merigo, D. Benati, M. Galie, C. Crescimanno, F. Osculati, and A. Sbarbati Immunohistochemical Localization of Cystic Fibrosis Transmembrane Regulator and Clara Cell Secretory Protein in Taste Receptor Cells of Rat Circumvallate Papillae Chem Senses, March 1, 2008; 33(3): 231 - 241. [Abstract] [Full Text] [PDF]

				H. Wang, M. Zhou, J. Brand, and L. Huang Inflammation Activates the Interferon Signaling Pathways in Taste Bud Cells J. Neurosci., October 3, 2007; 27(40): 10703 - 10713. [Abstract] [Full Text] [PDF]

				S. Busetto, E. Trevisan, E. Decleva, P. Dri, and R. Menegazzi Chloride Movements in Human Neutrophils during Phagocytosis: Characterization and Relationship to Granule Release J. Immunol., September 15, 2007; 179(6): 4110 - 4124. [Abstract] [Full Text] [PDF]

				J. A. DeSimone and V. Lyall Taste Receptors in the Gastrointestinal Tract III. Salty and sour taste: sensing of sodium and protons by the tongue Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1005 - G1010. [Abstract] [Full Text] [PDF]

				B. J. Canning, D. G. Farmer, and N. Mori Mechanistic studies of acid-evoked coughing in anesthetized guinea pigs Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R454 - R463. [Abstract] [Full Text] [PDF]