HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 43, 36380-36385, October 28, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/43/36380    most recent M506006200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Redon, E. Articles by Cocaign-Bousquet, M. Search for Related Content PubMed PubMed Citation Articles by Redon, E. Articles by Cocaign-Bousquet, M. Social Bookmarking                      What's this?

Role of mRNA Stability during Genome-wide Adaptation of Lactococcus lactis to Carbon Starvation^*

From the Laboratoire Biotechnologie Bioprocédés, Unité Mixte de Recherche 5504 CNRS and Unité Mixte de Recherche 792 Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées, 135 Avenue de Rangueil, 31077 Toulouse Cedex 4, France

The stability of mRNA was investigated for the first time at the genomic scale during carbon starvation adaptation of Lactococcus lactis IL1403. In exponential phase, mRNA half-lives were correlated positively to open reading frame length. A polypurine sequence, AGGAG, was identified as a putative 5'-stabilizer and inverted repeated sequences as a 3'-destabilizer. These original findings suggested that multiple pathways of mRNA degradation should coexist: internal cleavage, endonuclease cleavage initiated at the 5'-end, and exonuclease attack at the 3'-end. During carbon starvation adaptation, mRNA stability globally increased, but specific mechanisms allowing a wide range of stabilization factors between genes and differential kinetic evolution were involved. A formal method allowing the quantification of the relative influences of transcription and degradation on the mRNA pool control was developed and applied in L. lactis. Gene expression was mostly controlled by altered transcription prior to carbon source exhaustion, while the influence of mRNA stability increased during the starvation phase. This study highlighted that stability modulation in response to adverse growth conditions can govern gene regulation to the same extent as transcription in bacteria.

Received for publication, June 2, 2005 , and in revised form, August 29, 2005.

^* This work was supported by a grant from the Toulouse Génopole. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 33-561-559-438; Fax: 33-561-559-400; E-mail: cocaign{at}insa-toulouse.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. W. Y. Lau, R. G. Keil, and E. V. Armbrust Succession and Diel Transcriptional Response of the Glycolate-Utilizing Component of the Bacterial Community during a Spring Phytoplankton Bloom Appl. Envir. Microbiol., April 15, 2007; 73(8): 2440 - 2450. [Abstract] [Full Text] [PDF]

				H. Maamar, L. Abdou, C. Boileau, O. Valette, and C. Tardif Transcriptional Analysis of the cip-cel Gene Cluster from Clostridium cellulolyticum. J. Bacteriol., April 1, 2006; 188(7): 2614 - 2624. [Abstract] [Full Text] [PDF]