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J. Biol. Chem., Vol. 280, Issue 44, 36545-36550, November 4, 2005
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1
From the
Department of Integrative Biology and the Department of Molecular and Cellular Biology, University of Guelph, Ontario N1G 2W1, Canada
Na+-K+-ATPase is arguably the most important enzyme in the animal cell plasma membrane, but the role of the membrane in its regulation is poorly understood. We investigated the relationship between Na+-K+-ATPase and membrane microdomains or "lipid rafts" enriched in sulfatide (sulfogalactosylceramide/SGC), a glycosphingolipid implicated as a cofactor for this enzyme, in the basolateral membrane of rainbow trout gill epithelium. Our studies demonstrated that when trout adapt to seawater (33 ppt), Na+-K+-ATPase relocates to these structures. Arylsulfatase-induced desulfation of basolateral membrane SGC prevented this relocation and significantly reduced Na+-K+-ATPase activity in seawater but not freshwater trout. We contend that Na+-K+-ATPase partitions into SGC-enriched rafts to help facilitate the up-regulation of its activity during seawater adaptation. We also suggest that differential partitioning of Na+-K+-ATPase between these novel SGC-enriched regulatory platforms results in two distinct, physiological Na+ transport modes. In addition, we extend the working definition of cholesterol-dependent raft integrity to structural dependence on the sulfate moiety of SGC in this membrane.
Received for publication, June 20, 2005 , and in revised form, July 13, 2005.
* This work was supported by a National Sciences and Engineering Research Council of Canada Postgraduate Scholarship (to D. L.), a NSERC Discovery grant (to J. S. B.), and an operating grant from the Multiple Sclerosis Society of Canada (to G. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 To whom correspondence should be addressed: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. E-mail: lingwood{at}mpi-cbg.de
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