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J. Biol. Chem., Vol. 280, Issue 44, 36792-36801, November 4, 2005

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Anthocyanins Induce Cholesterol Efflux from Mouse Peritoneal Macrophages

THE ROLE OF THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR -LIVER X RECEPTOR -ABCA1 PATHWAY^*

From the Department of Nutrition, School of Public Health, Sun Yat-sen University (Northern Campus), 74 Zhongshan Road 2, Guangzhou, Guangdong Province 510080, China

It is widely accepted that stimulation of reverse cholesterol transport, the efflux of excess cholesterol from peripheral tissues and transferring it to the liver for biliary excretion, is becoming an important component in reducing excess cholesterol deposition in atherosclerotic plaques. The ATP-binding cassette transporter has been identified as a key regulator of macrophage cholesterol efflux and apoAI-mediated reverse cholesterol transport. In vivo studies have documented anthocyanins, a large group of naturally phenolic compounds rich in plants, possess substantial capacities in improving plasma cholesterol levels. In this study, we investigated the potential role of anthocyanins in modulating cholesterol efflux from mouse peritoneal macrophages and macrophage-derived foam cells and the possible molecular mechanism linking ABCA1 to cholesterol efflux. Incubation of the mouse peritoneal macrophages and macrophage-derived foam cells with cyanidin-3-O--glucoside and peonidin-3-O--glucoside led to dose-dependent (1–100 µM) induction in cholesterol efflux and ABCA1 mRNA expression, and this effect could be blocked by the ABCA1 inhibitor 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid, disodium salt, and a general inhibitor of gene transcription actinomycin D. Treatment of the macrophages with anthocyanins also activated peroxisome proliferator-activated receptor , liver X receptor mRNA expression, and their mediated gene expression. Addition of geranylgeranyl pyrophosphate ammonium salt or GW9662 markedly inhibited the anthocyanin-induced increase of ABCA1 gene expression and apoAI-mediated cholesterol efflux. These data demonstrated that anthocyanin induces cholesterol efflux from mouse peritoneal macrophages and macrophage-derived foam cells and that stimulation of cholesterol efflux by anthocyanin is mediated, at least in part, by peroxisome proliferator-activated receptor -liver X receptor -ABCA1 signaling pathway activation.

Received for publication, May 9, 2005 , and in revised form, August 16, 2005.

^* This work was supported by National Natural Science Foundation of China Research Grants 30025037 and 30371215 and China Medical Board of New York Inc. Grant CMB 98-677. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Nutrition, School of Public Health, Sun Yat-sen University (Northern Campus), Zhongshan Road 2, Guangzhou, Guangdong Province 510080, China. Tel.: 86-20-87331597; Fax: 86-20-87330446; E-mail: whling{at}gzsums.edu.cn.

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