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J. Biol. Chem., Vol. 280, Issue 45, 37717-37724, November 11, 2005

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Interaction with the Na,K-ATPase and Tissue Distribution of FXYD5 (Related to Ion Channel)^*

Irina Lubarski, Kaarina Pihakaski-Maunsbach, Steven J. D. Karlish1, Arvid B. Maunsbach, and Haim Garty, Incumbent of the Hella and Derrick Kleeman Chair of Biochemistry2

From the Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel and The Water and Salt Research Center, University of Aarhus, DK-8000 Aarhus, Denmark

FXYD5 (related to ion channel, dysadherin) is a member of the FXYD family of single span type I membrane proteins. Five members of this group have been shown to interact with the Na,K-ATPase and to modulate its properties. However, FXYD5 is structurally different from other family members and has been suggested to play a role in regulating E-cadherin and promoting metastasis (Ino, Y., Gotoh, M., Sakamoto, M., Tsukagoshi, K., and Hirohashi, S. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 365–370). The goal of this study was to determine whether FXYD5 can modulate the Na,K-ATPase activity, establish its cellular and tissue distribution, and characterize its biochemical properties. Anti-FXYD5 antibodies detected a 24-kDa polypeptide that was preferentially expressed in kidney, intestine, spleen, and lung. In kidney, FXYD5 resides in the basolateral membrane of the connecting tubule, the collecting tubule, and the intercalated cells of the collecting duct. However, there is also labeling of the apical membrane in long thin limb of Henle's loop. FXYD5 was effectively immunoprecipitated by antibodies to the subunit of Na,K-ATPase and the anti-FXYD5 antibody immunoprecipitates . Co-expressing FXYD5 with the 1 and 1 subunits of the Na,K-ATPase in Xenopus oocytes elicited a more than 2-fold increase in pump activity, measured either as ouabain-blockable outward current or as ouabain-sensitive ⁸⁶Rb⁺ uptake. Thus, as found with other FXYD proteins, FXYD5 interacts with the Na,K-ATPase and modulates its properties.

Received for publication, June 13, 2005 , and in revised form, August 29, 2005.

^* This work was supported by research grants from the Josef Cohen Minerva Center for Biomembranes and the Israel Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Incumbent of the William Smithburg Chair of Biochemistry.

² To whom correspondence should be addressed: Dept. of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel. Tel.: 972-8-9342706; Fax: 972-8-9344177; E-mail: h.garty{at}weizmann.ac.il.

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