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J. Biol. Chem., Vol. 280, Issue 45, 38096-38101, November 11, 2005

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In Vitro Murein (Peptidoglycan) Synthesis by Dimers of the Bifunctional Transglycosylase-Transpeptidase PBP1B from Escherichia coli^*

Ute Bertsche, Eefjan Breukink, Thomas Kast¶1, and Waldemar Vollmer2

From the Mikrobielle Genetik, Universität Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany, Department of Biochemistry of Membranes, Center of Biomembranes and Lipid Enzymology, Institute for Biomembranes, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands, and ^¶Max-Planck-Institut für Entwicklungsbiologie, Abteilung Biochemie, Spemannstrasse 35, 72076 Tübingen, Germany

PBP1B is a major bifunctional murein (peptidoglycan) synthase catalyzing transglycosylation and transpeptidation reactions in Escherichia coli. PBP1B has been shown to form dimers in vivo. The K_D value for PBP1B dimerization was determined by surface plasmon resonance. The effect of the dimerization of PBP1B on its activities was studied with a newly developed in vitro murein synthesis assay with radioactively labeled lipid II precursor as substrate. Under conditions at which PBP1B dimerizes, the enzyme synthesized murein with long glycan strands (>25 disaccharide units) and with almost 50% of the peptides being part of cross-links. PBP1B was also capable of synthesizing trimeric muropeptide structures. Tri-, tetra-, and pentapeptide compounds could serve as acceptors in the PBP1B-catalyzed transpeptidation reaction.

Received for publication, August 5, 2005 , and in revised form, September 8, 2005.

^* This project was supported by the Deutsche Forschungsgemeinschaft within the Forschergruppe Bakterielle Zellhülle (FOR 449) and by the European Commission within the SANITAS (QLK3-CT-2000-00079) and EUR-INTAFAR (LSHM-CT-2004-512138) networks. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Present address: Thieme Verlag, Rüdigerstrasse 14, 70469 Stuttgart, Germany.

² To whom correspondence should be addressed: Microbial Genetics, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany. Tel.: 49-7071-2974635; Fax: 49-7071-295065; E-mail: waldemar.vollmer{at}uni-tuebingen.de.

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