HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 46, 38290-38296, November 18, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/46/38290    most recent M507700200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fernández-Murray, J. P. Articles by McMaster, C. R. Search for Related Content PubMed PubMed Citation Articles by Fernández-Murray, J. P. Articles by McMaster, C. R. Social Bookmarking                      What's this?

Glycerophosphocholine Catabolism as a New Route for Choline Formation for Phosphatidylcholine Synthesis by the Kennedy Pathway^*

From the Departments of Pediatrics and Biochemistry and Molecular Biology, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada

In eukaryotes, neuropathy target esterase (Nte1p in yeast) deacylates phosphatidylcholine derived exclusively from the CDP-choline pathway to produce glycerophosphocholine (GroPCho) and release two fatty acids. The metabolic fate of GroPCho in eukaryotic cells is currently not known. Saccharomyces cerevisiae contains two open reading frames predicted to contain glycerophosphodiester phosphodiesterase domains, YPL110c and YPL206c. Pulse-chase experiments were conducted to monitor GroPCho metabolic fate under conditions known to alter CDP-choline pathway flux and consequently produce different rates of formation of GroPCho. From this analysis, it was revealed that GroPCho was metabolized to choline, with this choline serving as substrate for renewed synthesis of phosphatidylcholine. YPL110c played the major role in this metabolic pathway. To extend and confirm the metabolic studies, the ability of the ypl110c and ypl206c strains to utilize exogenous GroPCho or glycerophosphoinositol as the sole source of phosphate was analyzed. Consistent with our metabolic profiling, the ypl206c strain grew on both substrates with a similar rate to wild type, whereas the ypl110c strain grew very poorly on GroPCho and with moderately reduced growth on glycerophosphoinositol.

Received for publication, July 15, 2005 , and in revised form, September 16, 2005.

^* This work was supported by an operating grant from the Canadian Institutes of Health Research and a Canada Research Chair award. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Atlantic Research Centre, Dalhousie University, 5849 University Ave., Rm. C302, Halifax, Nova Scotia B3H 4H7, Canada. Tel.: 902-494-2953; Fax: 902-494-1394; E-mail: Christopher.mcmaster{at}dal.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Gallazzini, J. D. Ferraris, and M. B. Burg GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC PNAS, August 5, 2008; 105(31): 11026 - 11031. [Abstract] [Full Text] [PDF]

				K. Stalberg, A. C. Neal, H. Ronne, and U. Stahl Identification of a novel GPCAT activity and a new pathway for phosphatidylcholine biosynthesis in S. cerevisiae J. Lipid Res., August 1, 2008; 49(8): 1794 - 1806. [Abstract] [Full Text] [PDF]

				M. Simockova, R. Holic, D. Tahotna, J. Patton-Vogt, and P. Griac Yeast Pgc1p (YPL206c) Controls the Amount of Phosphatidylglycerol via a Phospholipase C-type Degradation Mechanism J. Biol. Chem., June 20, 2008; 283(25): 17107 - 17115. [Abstract] [Full Text] [PDF]

				V. Meyer, R. A. Damveld, M. Arentshorst, U. Stahl, C. A. M. J. J. van den Hondel, and A. F. J. Ram Survival in the Presence of Antifungals: GENOME-WIDE EXPRESSION PROFILING OF ASPERGILLUS NIGER IN RESPONSE TO SUBLETHAL CONCENTRATIONS OF CASPOFUNGIN AND FENPROPIMORPH J. Biol. Chem., November 9, 2007; 282(45): 32935 - 32948. [Abstract] [Full Text] [PDF]

				H. A. Boumann, J. Gubbens, M. C. Koorengevel, C.-S. Oh, C. E. Martin, A. J.R. Heck, J. Patton-Vogt, S. A. Henry, B. de Kruijff, and A. I.P.M. de Kroon Depletion of Phosphatidylcholine in Yeast Induces Shortening and Increased Saturation of the Lipid Acyl Chains: Evidence for Regulation of Intrinsic Membrane Curvature in a Eukaryote Mol. Biol. Cell, February 1, 2006; 17(2): 1006 - 1017. [Abstract] [Full Text] [PDF]