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J. Biol. Chem., Vol. 280, Issue 47, 39238-39245, November 25, 2005

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Glycine Metabolism in Intact Leaves by in Vivo 13C and ¹⁵N Labeling^*

From the Department of Chemistry, Washington University, St. Louis, Missouri 63130

Solid-state ¹³C NMR measurements of intact soybean leaves labeled by ¹³CO₂ (at subambient concentrations) show that excess glycine from the photorespiratory C₂ cycle (i.e. glycine not part of the production of glycerate in support of photosynthesis) is either fully decarboxylated or inserted as ¹³C-labeled glycyl residues in proteins. This ¹³C incorporation in leaf protein, which is uniformly ¹⁵N labeled by ¹⁵NH₄¹⁵NO₃, occurs as soon as 2 min after the start of ¹³CO₂ labeling. In those leaves with lower levels of available nitrogen (as measured by leaf nitrate and glutamine-glutamate concentrations), the excess glycine is used primarily as glycyl residues in protein.

Received for publication, June 28, 2005 , and in revised form, August 29, 2005.

^* This work was supported by the National Science Foundation under Grant MCB-0089905. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Chemistry, Washington University, 1 Brookings Dr., St. Louis, MO 63130. Tel.: 314-935-6844; Fax: 314-935-4481; E-mail: jschaefer{at}wustl.edu.

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