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J. Biol. Chem., Vol. 280, Issue 48, 39835-39842, December 2, 2005

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Glycosylphosphatidylinositol-anchored Fungal Polysaccharide in Aspergillus fumigatus^*

Corina Costachel, Bernadette Coddeville, Jean-Paul Latgé, and Thierry Fontaine1

From the Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France and the Laboratoire de Chimie Biologique, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France

Galactomannan is a characteristic polysaccharide of the human filamentous fungal pathogen Aspergillus fumigatus that can be used to diagnose invasive aspergillosis. In this study, we report the isolation of a galactomannan fraction associated to membrane preparations from A. fumigatus mycelium by a lipid anchor. Specific chemical and enzymatic degradations and mass spectrometry analysis showed that the lipid anchor is a glycosylphosphatidylinositol (GPI). The lipid part is an inositol phosphoceramide containing mainly C₁₈-phytosphingosine and monohydroxylated lignoceric acid (2OH-C_24:0 fatty acid). GPI glycan is a tetramannose structure linked to a glucosamine residue: Man1–2Man1–2Man1–6Man1–4GlcN. The galactomannan polymer is linked to the GPI structure throught the mannan chain. The GPI structure is a type 1, closely related to the one previously described for the GPI-anchored proteins of A. fumigatus. This is the first time that a fungal polysaccharide is shown to be GPI-anchored.

Received for publication, September 15, 2005 , and in revised form, October 4, 2005.

^* This work was supported in part by a research and development grant awarded by Aventis-Pharma (to J.-P. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 33-1-45-68-82-25; E-mail: tfontain{at}pasteur.fr.

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