HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 50, 41628-41635, December 16, 2005

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 280/50/41628    most recent M504497200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schweitzer, J. K. Articles by D'Souza-Schorey, C. Search for Related Content PubMed PubMed Citation Articles by Schweitzer, J. K. Articles by D'Souza-Schorey, C. Social Bookmarking                      What's this?

Endocytosis Resumes during Late Mitosis and Is Required for Cytokinesis^*

Jill Kuglin Schweitzer¶1, Erin E. Burke, Holly V. Goodson¶, and Crislyn D'Souza-Schorey¶2

From the Departments of Biological Sciences, Chemistry and Biochemistry, and the ^¶Walther Cancer Center, University of Notre Dame, Notre Dame, Indiana 46556-0369

Recent work has underscored the importance of membrane trafficking events during cytokinesis. For example, targeted membrane secretion occurs at the cleavage furrow in animal cells, and proteins that regulate endocytosis also influence the process of cytokinesis. Nonetheless, the prevailing dogma is that endosomal membrane trafficking ceases during mitosis and resumes after cell division is complete. In this study, we have characterized endocytic membrane trafficking events that occur during mammalian cell cytokinesis. We have found that, although endocytosis ceases during the early stages of mitosis, it resumes during late mitosis in a temporally and spatially regulated pattern as cells progress from anaphase to cytokinesis. Using fixed and live cell imaging, we have found that, during cleavage furrow ingression, vesicles are internalized from the polar region and subsequently trafficked to the midbody area during later stages of cytokinesis. In addition, we have demonstrated that cytokinesis is inhibited when clathrin-mediated endocytosis is blocked using a series of dominant negative mutants. In contrast to previous thought, we conclude that endocytosis resumes during the later stages of mitosis, before cytokinesis is completed. Furthermore, based on our findings, we propose that the proper regulation of endosomal membrane traffic is necessary for the successful completion of cytokinesis.

Received for publication, April 25, 2005 , and in revised form, September 26, 2005.

^* This work was supported in part by grants from the United States Department of Defense and the American Cancer Society (RSG 03-023-01-CSM) (to C. D-S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1-7, of which supplemental Figs. 3-6 consist of both still images and videos.

¹ Recipient of a Special Fellow Career Development Award from The Leukemia and Lymphoma Society.

² To whom correspondence should be addressed: Dept. of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369. Tel.: 574-631-3735; Fax: 574-631-7413; E-mail: D'Souza-Schorey.1{at}nd.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Zweifel, J. Smith, D. Romero, T. H. Giddings Jr., M. Winey, J. Honts, J. Dahlseid, B. Schneider, and E. S. Cole Nested Genes CDA12 and CDA13 Encode Proteins Associated with Membrane Trafficking in the Ciliate Tetrahymena thermophila Eukaryot. Cell, June 1, 2009; 8(6): 899 - 912. [Abstract] [Full Text] [PDF]

				J. W. Goss and D. K. Toomre Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis J. Cell Biol., October 22, 2008; 181(7): 1047 - 1054. [Abstract] [Full Text] [PDF]

				M. L. Wallander, K. B. Zumbrennen, E. S. Rodansky, S. J. Romney, and E. A. Leibold Iron-independent Phosphorylation of Iron Regulatory Protein 2 Regulates Ferritin during the Cell Cycle J. Biol. Chem., August 29, 2008; 283(35): 23589 - 23598. [Abstract] [Full Text] [PDF]

				R. Prekeris and G. W. Gould Breaking up is hard to do - membrane traffic in cytokinesis J. Cell Sci., May 15, 2008; 121(10): 1569 - 1576. [Abstract] [Full Text] [PDF]

				M. Onishi, M. Iida, T. Koga, S. Yamada, A. Hirata, T. Iwaki, K. Takegawa, Y. Fukui, and H. Tachikawa Schizosaccharomyces pombe Sst4p, a Conserved Vps27/Hrs Homolog, Functions Downstream of Phosphatidylinositol 3-Kinase Pik3p To Mediate Proper Spore Formation Eukaryot. Cell, December 1, 2007; 6(12): 2343 - 2353. [Abstract] [Full Text] [PDF]

				S. D. Arden, C. Puri, J. S.-Y. Au, J. Kendrick-Jones, and F. Buss Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis Mol. Biol. Cell, December 1, 2007; 18(12): 4750 - 4761. [Abstract] [Full Text] [PDF]

				M. G. Giansanti, G. Belloni, and M. Gatti Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males Mol. Biol. Cell, December 1, 2007; 18(12): 5034 - 5047. [Abstract] [Full Text] [PDF]

				L. Lanzetti, V. Margaria, F. Melander, L. Virgili, M.-H. Lee, J. Bartek, and S. Jensen Regulation of the Rab5 GTPase-activating Protein RN-tre by the Dual Specificity Phosphatase Cdc14A in Human Cells J. Biol. Chem., May 18, 2007; 282(20): 15258 - 15270. [Abstract] [Full Text] [PDF]

				E. Boucrot and T. Kirchhausen Endosomal recycling controls plasma membrane area during mitosis PNAS, May 8, 2007; 104(19): 7939 - 7944. [Abstract] [Full Text] [PDF]

				K. Kasahara, Y. Nakayama, Y. Nakazato, K. Ikeda, T. Kuga, and N. Yamaguchi Src Signaling Regulates Completion of Abscission in Cytokinesis through ERK/MAPK Activation at the Midbody J. Biol. Chem., February 23, 2007; 282(8): 5327 - 5339. [Abstract] [Full Text] [PDF]