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J. Biol. Chem., Vol. 280, Issue 6, 4975-4982, February 11, 2005

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Histone H2A Ubiquitination Does Not Preclude Histone H1 Binding, but It Facilitates Its Association with the Nucleosome^*

Laure J. M. Jason, Ron M. Finn¶, George Lindsey, and Juan Ausió¶||

From the Biacore Incorporated, Piscataway, New Jersey 08854, the Department of Molecular and Cell Biology, University of Cape Town, Private Bag Rondebosch 7701, South Africa, and the ^¶Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada

Histone H2A ubiquitination is a bulky posttranslational modification that occurs at the vicinity of the binding site for linker histones in the nucleosome. Therefore, we took several experimental approaches to investigate the role of ubiquitinated H2A (uH2A) in the binding of linker histones. Our results showed that uH2A was present in situ in histone H1-containing nucleosomes. Notably in vitro experiments using nucleosomes reconstituted onto 167-bp random sequence and 208-bp (5 S rRNA gene) DNA fragments showed that ubiquitination of H2A did not prevent binding of histone H1 but it rather enhanced the binding of this histone to the nucleosome. We also showed that ubiquitination of H2A did not affect the positioning of the histone octamer in the nucleosome in either the absence or the presence of linker histones.

Received for publication, September 7, 2004 , and in revised form, October 29, 2004.

This paper is dedicated to the memory of Robert T. Simpson.

^* This work was supported by a Foundation for Research Development research grant and a University of Cape Town research grant fund (to G. L.) and by Canadian Institute for Health Research Grant MOP-57718 (to J. A.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^|| To whom all correspondence should be addressed: Dept. of Biochemistry and Microbiology, University of Victoria, Petch Bldg., Rm. 220, Victoria, British Columbia V8N 5Y2, Canada. Tel.: 250-721-8863; Fax: 250-721-8855; E-mail: jausio{at}uvic.ca.

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