HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 6, 5013-5021, February 11, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/6/5013    most recent M409029200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marson, A. Articles by Kielty, C. M. Search for Related Content PubMed PubMed Citation Articles by Marson, A. Articles by Kielty, C. M. Social Bookmarking                      What's this?

Homotypic Fibrillin-1 Interactions in Microfibril Assembly^*

Andrew Marson, Matthew J. Rock, Stuart A. Cain, Lyle J. Freeman, Amanda Morgan, Kieran Mellody, C. Adrian Shuttleworth, Clair Baldock, and Cay M. Kielty

From the Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom

We have defined the homotypic interactions of fibrillin-1 to obtain new insights into microfibril assembly. Dose-dependent saturable high affinity binding was demonstrated between N-terminal fragments, between furin processed C-terminal fragments, and between these N- and C-terminal fragments. The N terminus also interacted with a downstream fragment. A post-furin cleavage site C-terminal sequence also interacted with the N terminus, with itself and with the furin-processed fragment. No other homotypic fibrillin-1 interactions were detected. Some terminal homotypic interactions were inhibited by other terminal sequences, and were strongly calcium-dependent. Treatment of an N-terminal fragment with N-ethylmaleimide reduced homotypic binding. Microfibril-associated glycoprotein-1 inhibited N- to C-terminal interactions but not homotypic N-terminal interactions. These fibrillin-1 interactions are likely to regulate pericellular fibrillin-1 microfibril assembly.

Received for publication, August 6, 2004 , and in revised form, October 13, 2004.

^* This work was supported by the Medical Research Council (UK), the Biotechnology and Biological Sciences Research Council (UK), European Union Grant QLK6-CT-2001-00332, and a grant from the Royal Society (to C. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Bldg., Oxford Road, Manchester M13 9PT, United Kingdom. Tel.: 44-161-275-5739; Fax: 44-161-275-5082; E-mail: cay.kielty{at}man.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Sabatier, D. Chen, C. Fagotto-Kaufmann, D. Hubmacher, M. D. McKee, D. S. Annis, D. F. Mosher, and D. P. Reinhardt Fibrillin Assembly Requires Fibronectin Mol. Biol. Cell, February 1, 2009; 20(3): 846 - 858. [Abstract] [Full Text] [PDF]

				S. A. Cain, A. K. Baldwin, Y. Mahalingam, B. Raynal, T. A. Jowitt, C. A. Shuttleworth, J. R. Couchman, and C. M. Kielty Heparan Sulfate Regulates Fibrillin-1 N- and C-terminal Interactions J. Biol. Chem., October 3, 2008; 283(40): 27017 - 27027. [Abstract] [Full Text] [PDF]

				R. Kinsey, M. R. Williamson, S. Chaudhry, K. T. Mellody, A. McGovern, S. Takahashi, C. A. Shuttleworth, and C. M. Kielty Fibrillin-1 microfibril deposition is dependent on fibronectin assembly J. Cell Sci., August 15, 2008; 121(16): 2696 - 2704. [Abstract] [Full Text] [PDF]

				D. Hubmacher, E. I. El-Hallous, V. Nelea, M. T. Kaartinen, E. R. Lee, and D. P. Reinhardt Biogenesis of extracellular microfibrils: Multimerization of the fibrillin-1 C terminus into bead-like structures enables self-assembly PNAS, May 6, 2008; 105(18): 6548 - 6553. [Abstract] [Full Text] [PDF]

				C. M Kielty, S. Stephan, M. J Sherratt, M. Williamson, and C. A. Shuttleworth Applying elastic fibre biology in vascular tissue engineering Phil Trans R Soc B, August 29, 2007; 362(1484): 1293 - 1312. [Abstract] [Full Text] [PDF]

				E. El-Hallous, T. Sasaki, D. Hubmacher, M. Getie, K. Tiedemann, J. Brinckmann, B. Batge, E. C. Davis, and D. P. Reinhardt Fibrillin-1 Interactions with Fibulins Depend on the First Hybrid Domain and Provide an Adaptor Function to Tropoelastin J. Biol. Chem., March 23, 2007; 282(12): 8935 - 8946. [Abstract] [Full Text] [PDF]

				S. S. Chaudhry, S. A. Cain, A. Morgan, S. L. Dallas, C. A. Shuttleworth, and C. M. Kielty Fibrillin-1 regulates the bioavailability of TGF{beta}1 J. Cell Biol., January 29, 2007; 176(3): 355 - 367. [Abstract] [Full Text] [PDF]

				P N Robinson, E Arteaga-Solis, C Baldock, G Collod-Beroud, P Booms, A De Paepe, H C Dietz, G Guo, P A Handford, D P Judge, et al. The molecular genetics of Marfan syndrome and related disorders J. Med. Genet., October 1, 2006; 43(10): 769 - 787. [Abstract] [Full Text] [PDF]

				C. Baldock, V. Siegler, D. V. Bax, S. A. Cain, K. T. Mellody, A. Marson, J. L. Haston, R. Berry, M.-C. Wang, J. G. Grossmann, et al. Nanostructure of fibrillin-1 reveals compact conformation of EGF arrays and mechanism for extensibility PNAS, August 8, 2006; 103(32): 11922 - 11927. [Abstract] [Full Text] [PDF]

				D. Hubmacher, K. Tiedemann, R. Bartels, J. Brinckmann, T. Vollbrandt, B. Batge, H. Notbohm, and D. P. Reinhardt Modification of the Structure and Function of Fibrillin-1 by Homocysteine Suggests a Potential Pathogenetic Mechanism in Homocystinuria J. Biol. Chem., October 14, 2005; 280(41): 34946 - 34955. [Abstract] [Full Text] [PDF]

				S. A. Cain, C. Baldock, J. Gallagher, A. Morgan, D. V. Bax, A. S. Weiss, C. A. Shuttleworth, and C. M. Kielty Fibrillin-1 Interactions with Heparin: IMPLICATIONS FOR MICROFIBRIL AND ELASTIC FIBER ASSEMBLY J. Biol. Chem., August 26, 2005; 280(34): 30526 - 30537. [Abstract] [Full Text] [PDF]