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J. Biol. Chem., Vol. 280, Issue 7, 5163-5168, February 18, 2005

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Pigment Binding, Fluorescence Properties, and Oligomerization Behavior of Lhca5, a Novel Light-harvesting Protein^*

Stefanie Storf, Stefan Jansson, and Volkmar H. R. Schmid¶

From the Institut für Allgemeine Botanik, Johannes Gutenberg-Universität Mainz, Müllerweg 6, 55099 Mainz, Germany and the Department of Plant Physiology, University of Umeå, S-901 87 Umeå, Sweden

A new potential light-harvesting protein, named Lhca5, was recently detected in higher plants. Because of the low amount of Lhca5 in thylakoid membranes, the isolation of a native Lhca5 pigment-protein complex has not been achieved to date. Therefore, we used in vitro reconstitution to analyze whether Lhca5 binds pigments and is actually an additional light-harvesting protein. By this approach we could demonstrate that Lhca5 binds pigments in a unique stoichiometry. Analyses of pigment requirements for light-harvesting complex formation by Lhca5 revealed that chlorophyll b is the only indispensable pigment. Fluorescence measurements showed that ligated chlorophylls and carotenoids are arranged in a way that allows directed energy transfer within the light-harvesting complex. Reconstitutions of Lhca5 together with other Lhca proteins resulted in the formation of heterodimers with Lhca1. This result demonstrates that Lhca5 is indeed a protein belonging to the light-harvesting antenna of photosystem I. The properties of Lhca5 are compared with those of previously characterized Lhca proteins, and the consequences of an additional Lhca protein for the composition of the light-harvesting antenna of photosystem I are discussed in view of the recently published photosystem I structure of the pea.

Received for publication, October 1, 2004 , and in revised form, November 23, 2004.

^* This project was funded Deutsche Forschungsmeinschaft Grant Schm 1203/3-1. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. Tel.: 49-6131-3924203; Fax: 49-6131-3923787; E-mail: vschmid{at}uni-mainz.de.

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