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J. Biol. Chem., Vol. 280, Issue 7, 6109-6117, February 18, 2005

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Huntingtin-interacting Protein 1 (Hip1) and Hip1-related Protein (Hip1R) Bind the Conserved Sequence of Clathrin Light Chains and Thereby Influence Clathrin Assembly in Vitro and Actin Distribution in Vivo^*

Chih-Ying Chen and Frances M. Brodsky

From the G. W. Hooper Foundation, Departments of Biopharmaceutical Sciences, Microbiology and Immunology, and Pharmaceutical Chemistry, University of California, San Francisco, California 94143-0552

Clathrin heavy and light chains form triskelia, which assemble into polyhedral coats of membrane vesicles that mediate transport for endocytosis and organelle biogenesis. Light chain subunits regulate clathrin assembly in vitro by suppressing spontaneous self-assembly of the heavy chains. The residues that play this regulatory role are at the N terminus of a conserved 22-amino acid sequence that is shared by all vertebrate light chains. Here we show that these regulatory residues and others in the conserved sequence mediate light chain interaction with Hip1 and Hip1R. These related proteins were previously found to be enriched in clathrin-coated vesicles and to promote clathrin assembly in vitro. We demonstrate Hip1R binding preference for light chains associated with clathrin heavy chain and show that Hip1R stimulation of clathrin assembly in vitro is blocked by mutations in the conserved sequence of light chains that abolish interaction with Hip1 and Hip1R. In vivo overexpression of a fragment of clathrin light chain comprising the Hip1R-binding region affected cellular actin distribution. Together these results suggest that the roles of Hip1 and Hip1R in affecting clathrin assembly and actin distribution are mediated by their interaction with the conserved sequence of clathrin light chains.

Received for publication, July 26, 2004 , and in revised form, October 7, 2004.

^* This work was supported by National Institutes of Health Grant GM38093 (to F. M. B.) and a postdoctoral fellowship from the Arthritis Foundation and National Institutes of Health Training Grant CA09043 (to C.-Y. C.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 415-476-6406; Fax: 415-476-6185; E-mail: fmarbro{at}itsa.ucsf.edu.

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