HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 8, 6655-6662, February 25, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/8/6655    most recent M413288200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, C. Articles by Yu, Y.-T. Search for Related Content PubMed PubMed Citation Articles by Yang, C. Articles by Yu, Y.-T. Social Bookmarking                      What's this?

35 in the Branch Site Recognition Region of U2 Small Nuclear RNA Is Important for Pre-mRNA Splicing in Saccharomyces cerevisiae^*

Chunxing Yang, David S. McPheeters, and Yi-Tao Yu¶

From the Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642 and the Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106

Pseudouridine 35 (35) in the branch site recognition region of yeast U2 small nuclear RNA is absolutely conserved in all eukaryotes examined. Pus7p catalyzes pseudouridylation at position 35 in Saccharomyces cerevisiae U2. The pus7 deletion strain, although viable in rich medium, is growth-disadvantaged under certain conditions. To clarify the function of U2 35 in yeast, we used this pus7 deletion strain to screen a collection of mutant U2 small nuclear RNAs, each containing a point mutation near the branch site recognition sequence, for a synthetic growth defect phenotype. The screen identified two U2 mutants, one containing a U40 G40 substitution (U40G) and another having a U40 deletion (U40). Yeast strains carrying either of these U2 mutations grew as well as the wild-type strain in the selection medium, but they exhibited a temperature-sensitive growth defect phenotype when coupled with the pus7 deletion (pus7). A subsequent temperature shift assay and a conditional pus7 depletion (via GAL promoter shutoff) in the U2-U40 mutant genetic background caused pre-mRNA accumulation, suggesting that 35 is required for pre-mRNA splicing under certain conditions.

Received for publication, November 24, 2004

^* This work was supported by National Institutes of Health Grants GM62937 (to Y.-T. Y.) and GM064682 (to D. S. McP.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed: Dept. of Biochemistry and Biophysics, University of Rochester Medical Center, 601 Elmwood Ave., Rochester, NY 14642. Tel.: 585-275-1271; Fax: 585-275-6007; E-mail: yitao_yu{at}urmc.rochester.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Muller, F. Leclerc, I. Behm-Ansmant, J.-B. Fourmann, B. Charpentier, and C. Branlant Combined in silico and experimental identification of the Pyrococcus abyssi H/ACA sRNAs and their target sites in ribosomal RNAs Nucleic Acids Res., May 1, 2008; 36(8): 2459 - 2475. [Abstract] [Full Text] [PDF]

				S. Muller, J.-B. Fourmann, C. Loegler, B. Charpentier, and C. Branlant Identification of determinants in the protein partners aCBF5 and aNOP10 necessary for the tRNA:{Psi}55-synthase and RNA-guided RNA:{Psi}-synthase activities Nucleic Acids Res., August 17, 2007; (2007) gkm606v1. [Abstract] [Full Text] [PDF]

				C. Normand, R. Capeyrou, S. Quevillon-Cheruel, A. Mougin, Y. Henry, and M. Caizergues-Ferrer Analysis of the binding of the N-terminal conserved domain of yeast Cbf5p to a box H/ACA snoRNA RNA, October 1, 2006; 12(10): 1868 - 1882. [Abstract] [Full Text] [PDF]

				X. Manival, C. Charron, J.-B. Fourmann, F. Godard, B. Charpentier, and C. Branlant Crystal structure determination and site-directed mutagenesis of the Pyrococcus abyssi aCBF5-aNOP10 complex reveal crucial roles of the C-terminal domains of both proteins in H/ACA sRNP activity Nucleic Acids Res., February 2, 2006; 34(3): 826 - 839. [Abstract] [Full Text] [PDF]

				B. Charpentier, S. Muller, and C. Branlant Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation Nucleic Acids Res., June 2, 2005; 33(10): 3133 - 3144. [Abstract] [Full Text] [PDF]