| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 280, Issue 9, 7793-7799, March 4, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the CNRS-UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, Lille 59021, France
The scavenger receptor class B type I (SR-BI) has recently been shown to interact with hepatitis C virus (HCV) envelope glycoprotein E2, suggesting that it might be involved at some step of HCV entry into host cells. However, due to the absence of a cell culture system to efficiently amplify HCV, it is not clear how SR-BI contributes to HCV entry. Here, we sought to determine how high density lipoproteins (HDLs), the natural ligand of SR-BI, affect HCV entry. By using the recently described infectious HCV pseudotyped particles (HCVpps) that display functional E1E2 glycoprotein complexes, we showed that HDLs are able to markedly enhance HCVpp entry. We did not find any evidence of HDL association with HCVpps, suggesting that HCVpps do not enter into target cells using HDL as a carrier to bind to its receptor. Interestingly, lipid-free apoA-I and apoA-II, the major HDL apolipoproteins, were unable to enhance HCVpp infectivity. In addition, drugs inhibiting HDL cholesteryl transfer (block lipid transport (BLT)-2 and BLT-4) reduced HDL enhancement of HCVpp entry, suggesting a role for lipid transfer in facilitating HCVpp entry. Importantly, silencing of SR-BI expression in target cells by RNA interference markedly reduced HDL-mediated enhancement of HCVpp entry. Finally, enhancement of HCVpp entry was also suppressed when the SR-BI binding region on HCV glycoprotein E2 was deleted. Altogether, these data indicate that HDL-mediated enhancement of HCVpp entry involves a complex interplay between SR-BI, HDL, and HCV envelope glycoproteins, and they highlight the active role of HDLs in HCV entry.
Received for publication, October 12, 2004 , and in revised form, December 14, 2004.
* This work was supported by grants from INSERM (ATC "hépatite C") and the Agence Nationale de Recherche sur le Sida et les Hépatites Virales and European Union Grants QLRT-2000-01120 and QLRT-2001-0132. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Supported by a CNRS post-doctoral fellowship.
Supported by an Agence Nationale de Recherche sur le Sida et les Hépatites Virales post-doctoral fellowship.
¶ Contributed equally to this study.
|| To whom correspondence should be addressed: Unité Hépatite C, CNRS-UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille cedex, France. Tel.: 33-3-20-87-11-60; Fax: 33-3-20-87-12-01; E-mail: jean.dubuisson{at}ibl.fr.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. J. Harris, M. J. Farquhar, C. J. Mee, C. Davis, G. M. Reynolds, A. Jennings, K. Hu, F. Yuan, H. Deng, S. G. Hubscher, et al. CD81 and Claudin 1 Coreceptor Association: Role in Hepatitis C Virus Entry J. Virol., May 15, 2008; 82(10): 5007 - 5020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jones and J. S Owen How does interferon inhibit HCV cell entry? Gut, May 1, 2008; 57(5): 573 - 574. [Full Text] [PDF]
|
|
|
|
|
|
K Murao, H Imachi, X Yu, W M Cao, T Nishiuchi, K Chen, J Li, R A M Ahmed, N C W Wong, and T Ishida Interferon {alpha} decreases expression of human scavenger receptor class BI, a possible HCV receptor in hepatocytes Gut, May 1, 2008; 57(5): 664 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Barth, E. K. Schnober, C. Neumann-Haefelin, C. Thumann, M. B. Zeisel, H. M. Diepolder, Z. Hu, T. J. Liang, H. E. Blum, R. Thimme, et al. Scavenger Receptor Class B Is Required for Hepatitis C Virus Uptake and Cross-Presentation by Human Dendritic Cells J. Virol., April 1, 2008; 82(7): 3466 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. von Hahn and C. M. Rice Hepatitis C Virus Entry J. Biol. Chem., February 15, 2008; 283(7): 3689 - 3693. [Full Text] [PDF]
|
|
|
|
|
|
A. Zheng, F. Yuan, Y. Li, F. Zhu, P. Hou, J. Li, X. Song, M. Ding, and H. Deng Claudin-6 and Claudin-9 Function as Additional Coreceptors for Hepatitis C Virus J. Virol., November 15, 2007; 81(22): 12465 - 12471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dreux, B. Boson, S. Ricard-Blum, J. Molle, D. Lavillette, B. Bartosch, E.-I. Pecheur, and F.-L. Cosset The Exchangeable Apolipoprotein ApoC-I Promotes Membrane Fusion of Hepatitis C Virus J. Biol. Chem., November 2, 2007; 282(44): 32357 - 32369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Tarr, A. M. Owsianka, D. Jayaraj, R. J. P. Brown, T. P. Hickling, W. L. Irving, A. H. Patel, and J. K. Ball Determination of the human antibody response to the epitope defined by the hepatitis C virus-neutralizing monoclonal antibody AP33 J. Gen. Virol., November 1, 2007; 88(11): 2991 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Tellinghuisen, M. J. Evans, T. von Hahn, S. You, and C. M. Rice Studying Hepatitis C Virus: Making the Best of a Bad Virus J. Virol., September 1, 2007; 81(17): 8853 - 8867. [Full Text] [PDF]
|
|
|
|
|
|
K. McCaffrey, I. Boo, P. Poumbourios, and H. E. Drummer Expression and Characterization of a Minimal Hepatitis C Virus Glycoprotein E2 Core Domain That Retains CD81 Binding J. Virol., September 1, 2007; 81(17): 9584 - 9590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. F. Nieland, J. T. Shaw, F. A. Jaipuri, Z. Maliga, J. L. Duffner, A. N. Koehler, and M. Krieger Influence of HDL-cholesterol-elevating drugs on the in vitro activity of the HDL receptor SR-BI J. Lipid Res., August 1, 2007; 48(8): 1832 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Catanese, R. Graziani, T. von Hahn, M. Moreau, T. Huby, G. Paonessa, C. Santini, A. Luzzago, C. M. Rice, R. Cortese, et al. High-Avidity Monoclonal Antibodies against the Human Scavenger Class B Type I Receptor Efficiently Block Hepatitis C Virus Infection in the Presence of High-Density Lipoprotein J. Virol., August 1, 2007; 81(15): 8063 - 8071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Akazawa, T. Date, K. Morikawa, A. Murayama, M. Miyamoto, M. Kaga, H. Barth, T. F. Baumert, J. Dubuisson, and T. Wakita CD81 Expression Is Important for the Permissiveness of Huh7 Cell Clones for Heterogeneous Hepatitis C Virus Infection J. Virol., May 15, 2007; 81(10): 5036 - 5045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chapel, C. Garcia, B. Bartosch, P. Roingeard, N. Zitzmann, F.-L. Cosset, J. Dubuisson, R. A. Dwek, C. Trepo, F. Zoulim, et al. Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitors J. Gen. Virol., April 1, 2007; 88(4): 1133 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Grove, T. Huby, Z. Stamataki, T. Vanwolleghem, P. Meuleman, M. Farquhar, A. Schwarz, M. Moreau, J. S. Owen, G. Leroux-Roels, et al. Scavenger Receptor BI and BII Expression Levels Modulate Hepatitis C Virus Infectivity J. Virol., April 1, 2007; 81(7): 3162 - 3169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Kapadia, H. Barth, T. Baumert, J. A. McKeating, and F. V. Chisari Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I J. Virol., January 1, 2007; 81(1): 374 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Vishnyakova, R. Kurlander, A. V. Bocharov, I. N. Baranova, Z. Chen, M. S. Abu-Asab, M. Tsokos, D. Malide, F. Basso, A. Remaley, et al. CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells PNAS, November 7, 2006; 103(45): 16888 - 16893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Voisset, A. O. de Beeck, P. Horellou, M. Dreux, T. Gustot, G. Duverlie, F.-L. Cosset, N. Vu-Dac, and J. Dubuisson High-density lipoproteins reduce the neutralizing effect of hepatitis C virus (HCV)-infected patient antibodies by promoting HCV entry J. Gen. Virol., September 1, 2006; 87(9): 2577 - 2581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Owsianka, J. M. Timms, A. W. Tarr, R. J. P. Brown, T. P. Hickling, A. Szwejk, K. Bienkowska-Szewczyk, B. J. Thomson, A. H. Patel, and J. K. Ball Identification of conserved residues in the e2 envelope glycoprotein of the hepatitis C virus that are critical for CD81 binding. J. Virol., September 1, 2006; 80(17): 8695 - 8704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Blanchard, S. Belouzard, L. Goueslain, T. Wakita, J. Dubuisson, C. Wychowski, and Y. Rouille Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis J. Virol., July 15, 2006; 80(14): 6964 - 6972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dreux, T. Pietschmann, C. Granier, C. Voisset, S. Ricard-Blum, P.-E. Mangeot, Z. Keck, S. Foung, N. Vu-Dac, J. Dubuisson, et al. High Density Lipoprotein Inhibits Hepatitis C Virus-neutralizing Antibodies by Stimulating Cell Entry via Activation of the Scavenger Receptor BI J. Biol. Chem., July 7, 2006; 281(27): 18285 - 18295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Silvie, S. Charrin, M. Billard, J.-F. Franetich, K. L. Clark, G.-J. van Gemert, R. W. Sauerwein, F. Dautry, C. Boucheix, D. Mazier, et al. Cholesterol contributes to the organization of tetraspanin-enriched microdomains and to CD81-dependent infection by malaria sporozoites J. Cell Sci., May 15, 2006; 119(10): 1992 - 2002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cocquerel, C. Voisset, and J. Dubuisson Hepatitis C virus entry: potential receptors and their biological functions. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1075 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Pagler, S. Rhode, A. Neuhofer, H. Laggner, W. Strobl, C. Hinterndorfer, I. Volf, M. Pavelka, E. R. M. Eckhardt, D. R. van der Westhuyzen, et al. SR-BI-mediated High Density Lipoprotein (HDL) Endocytosis Leads to HDL Resecretion Facilitating Cholesterol Efflux J. Biol. Chem., April 21, 2006; 281(16): 11193 - 11204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Lindenbach, P. Meuleman, A. Ploss, T. Vanwolleghem, A. J. Syder, J. A. McKeating, R. E. Lanford, S. M. Feinstone, M. E. Major, G. Leroux-Roels, et al. From the Cover: Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro PNAS, March 7, 2006; 103(10): 3805 - 3809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bukh and R. H. Purcell A milestone for hepatitis C virus research: A virus generated in cell culture is fully viable in vivo PNAS, March 7, 2006; 103(10): 3500 - 3501. [Full Text] [PDF]
|
|
|
|
|
|
D. M. Tscherne, C. T. Jones, M. J. Evans, B. D. Lindenbach, J. A. McKeating, and C. M. Rice Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered Entry J. Virol., February 15, 2006; 80(4): 1734 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Callens, Y. Ciczora, B. Bartosch, N. Vu-Dac, F.-L. Cosset, J.-M. Pawlotsky, F. Penin, and J. Dubuisson Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry J. Virol., December 15, 2005; 79(24): 15331 - 15341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Meunier, R. E. Engle, K. Faulk, M. Zhao, B. Bartosch, H. Alter, S. U. Emerson, F.-L. Cosset, R. H. Purcell, and J. Bukh Evidence for cross-genotype neutralization of hepatitis C virus pseudo-particles and enhancement of infectivity by apolipoprotein C1 PNAS, March 22, 2005; 102(12): 4560 - 4565. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
