HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 280, Issue 9, 8316-8323, March 4, 2005

This Article Full Text Full Text (PDF) All Versions of this Article: 280/9/8316    most recent M412730200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liao, H. Articles by Xiao, Z.-C. Search for Related Content PubMed PubMed Citation Articles by Liao, H. Articles by Xiao, Z.-C. Social Bookmarking                      What's this?

Tenascin-R Plays a Role in Neuroprotection via Its Distinct Domains That Coordinate to Modulate the Microglia Function^*

Hong Liao, Wen-yu Bu, Ting-Hua Wang¶, Sohail Ahmed, and Zhi-Cheng Xiao||**

From the Department of Clinical Research, Singapore General Hospital, Singapore 169609, Centre for Molecular Medicine, Singapore 117609, ^¶Department of Histology, Embryology and Neurobiology, College of Preclinical and Forensic Medicine, Sichuan University, Chendu, China 610065, and ^||Institute of Molecular and Cell Biology, Singapore 117609

Microglia are one of the main cell types activated by brain injury. In the present study, we have investigated how domains of the extracellular matrix molecule tenascin-R (TN-R) modulate microglia function. We found that epidermal growth factor-like repeats inhibited adhesion and migration of microglia via a protein kinase A-dependent mechanism. In contrast, fibronectin 6–8 repeats promoted adhesion and migration of the primary microglia via a protein kinase C-dependent mechanism. Both domains of TN-R induced an up-regulation in the secretion of cytokines, such as chemokine-induced cytokine 3 and tumor neurosis factor . Interestingly, epidermal growth factor-like repeats and fibronectin 6–8 induced a dramatic up-regulation in the secretion of brain-derived neurotrophic factor/transforming growth factor- and nerve growth factor/transforming growth factor-, respectively, and conditioned medium from activated microglia was able to promote neurite outgrowth of N1E-115 cells and primary cortical neurons. These results suggest that TN-R plays a role in neuroprotection through distinct domains coordinating to modulate microglia function.

Received for publication, November 10, 2004

^* This work was supported by grants from the National Medical Research Council of Singapore, Singapore Health Service Pte Ltd., and Department of Clinical Research, Singapore General Hospital (to Z.-C. X.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^** To whom correspondence should be addressed: Neurobiology Laboratory, Dept. of Clinical Research, Singapore General Hospital, Block A, No. 7 Hospital Dr., Singapore 169608. Tel.: 65-6326-6195; Fax: 65-6321-3606; E-mail: gcrxzc{at}sgh.com.sg.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Liao, W. Huang, M. Schachner, Y. Guan, J. Guo, J. Yan, J. Qin, X. Bai, and L. Zhang {beta} 1 Integrin-mediated Effects of Tenascin-R Domains EGFL and FN6-8 on Neural Stem/Progenitor Cell Proliferation and Differentiation in Vitro J. Biol. Chem., October 10, 2008; 283(41): 27927 - 27936. [Abstract] [Full Text] [PDF]

				M. H. DeLegge and A. Smoke Neurodegeneration and Inflammation Nutr Clin Pract, February 1, 2008; 23(1): 35 - 41. [Abstract] [Full Text] [PDF]

				I. Apostolova, A. Irintchev, and M. Schachner Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice. J. Neurosci., July 26, 2006; 26(30): 7849 - 7859. [Abstract] [Full Text] [PDF]

				H. C. Hsia and J. E. Schwarzbauer Meet the Tenascins: Multifunctional and Mysterious J. Biol. Chem., July 22, 2005; 280(29): 26641 - 26644. [Full Text] [PDF]