HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 1, 13-15, January 6, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/1/13    most recent C500423200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vojnic, E. Articles by Cramer, P. Search for Related Content PubMed PubMed Citation Articles by Vojnic, E. Articles by Cramer, P. Social Bookmarking                      What's this?

Structure and Carboxyl-terminal Domain (CTD) Binding of the Set2 SRI Domain That Couples Histone H3 Lys³⁶ Methylation to Transcription^*

Erika Vojnic, Bernd Simon, Brian D. Strahl¶1, Michael Sattler2, and Patrick Cramer3

From the Gene Center, Department of Chemistry and Biochemistry, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany, the EMBL, Meyerhofstrasse 1, 69120 Heidelberg, Germany, and the ^¶Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-7260

During mRNA elongation, the SRI domain of the histone H3 methyltransferase Set2 binds to the phosphorylated carboxyl-terminal domain (CTD) of RNA polymerase II. The solution structure of the yeast Set2 SRI domain reveals a novel CTD-binding fold consisting of a left-handed three-helix bundle. NMR titration shows that the SRI domain binds an Ser²/Ser⁵-phosphorylated CTD peptide comprising two heptapeptide repeats and three flanking NH₂-terminal residues, whereas a single CTD repeat is insufficient for binding. Residues that show strong chemical shift perturbations upon CTD binding cluster in two regions. Both CTD tyrosine side chains contact the SRI domain. One of the tyrosines binds in the region with the strongest chemical shift perturbations, formed by the two NH₂-terminal helices. Unexpectedly, the SRI domain fold resembles the structure of an RNA polymerase-interacting domain in bacterial factors (domain ₂ in ⁷⁰).

Received for publication, October 25, 2005 , and in revised form, November 10, 2005.

^* This work was supported in part by the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Table S1 and Figs. S1–S3.

The atomic coordinates and structure factors (code 2C5Z) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).

¹ Supported by the National Institutes of Health and is a Pew Scholar in the Biomedical Sciences.

² To whom correspondence may be addressed. E-mail: sattler{at}embl-heidelberg.de. ³ To whom correspondence may be addressed. Tel.: 49-89-2180-76951; Fax: 49-89-2180-76999; E-mail: cramer{at}LMB.uni-muenchen.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. M. Yoh, J. S. Lucas, and K. A. Jones The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation Genes & Dev., December 15, 2008; 22(24): 3422 - 3434. [Abstract] [Full Text] [PDF]

				M. L. Youdell, K. O. Kizer, E. Kisseleva-Romanova, S. M. Fuchs, E. Duro, B. D. Strahl, and J. Mellor Roles for Ctk1 and Spt6 in Regulating the Different Methylation States of Histone H3 Lysine 36 Mol. Cell. Biol., August 15, 2008; 28(16): 4915 - 4926. [Abstract] [Full Text] [PDF]

				P. Liu, A. L. Greenleaf, and J. W. Stiller The Essential Sequence Elements Required for RNAP II Carboxyl-terminal Domain Function in Yeast and Their Evolutionary Conservation Mol. Biol. Evol., April 1, 2008; 25(4): 719 - 727. [Abstract] [Full Text] [PDF]

				R. D. Chapman, M. Heidemann, T. K. Albert, R. Mailhammer, A. Flatley, M. Meisterernst, E. Kremmer, and D. Eick Transcribing RNA Polymerase II Is Phosphorylated at CTD Residue Serine-7 Science, December 14, 2007; 318(5857): 1780 - 1782. [Abstract] [Full Text] [PDF]

				H. P. Phatnani and A. L. Greenleaf Phosphorylation and functions of the RNA polymerase II CTD. Genes & Dev., November 1, 2006; 20(21): 2922 - 2936. [Abstract] [Full Text] [PDF]