HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 11, 7332-7340, March 17, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/11/7332    most recent M511857200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gaultier, A. Articles by Gonias, S. L. Search for Related Content PubMed PubMed Citation Articles by Gaultier, A. Articles by Gonias, S. L. Social Bookmarking                      What's this?

Regulation of the Composition of the Extracellular Matrix by Low Density Lipoprotein Receptor-related Protein-1

ACTIVITIES BASED ON REGULATION OF mRNA EXPRESSION^*

Alban Gaultier, Ana Maria Salicioni, Sanja Arandjelovic, and Steven L. Gonias¹

From the Department of Pathology, University of California San Diego School of Medicine, La Jolla, California 92093 and the Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003

Low density lipoprotein receptor-related protein-1 (LRP-1) is a catabolic receptor for extracellular matrix (ECM) structural proteins and for proteins that bind to ECM. LRP-1 also is implicated in integrin maturation. In this study, we applied a proteomics strategy to identify novel proteins involved in ECM modeling that are regulated by LRP-1. We show that LRP-1 deficiency in murine embryonic fibroblasts (MEFs) is associated with increased levels of type III collagen and pigment epithelium-derived factor, which accumulate in the substratum surrounding cells. The collagen receptor, uPAR-AP/Endo-180, is also increased in LRP-1-deficient MEFs. Human LRP-1 reversed the changes in protein expression associated with LRP-1 deficiency; however, the endocytic activity of LRP-1 was not involved. Instead, regulation occurred at the mRNA level. Inhibition of c-Jun amino-terminal kinase (JNK) blocked type III collagen expression in LRP-1-deficient MEFs, suggesting regulation of JNK activity as a mechanism by which LRP-1 controls mRNA expression. The ability of LRP-1 to regulate expression of the factors identified here suggests a role for LRP-1 in determining blood vessel structure and in angiogenesis.

Received for publication, November 3, 2005

^* This work was supported by Grant R01 HL60551 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Pathology, UCSD School of Medicine, 9500 Gilman Dr., La Jolla, CA 92093. Tel.: 858-534-1887; Fax: 858-534-0414; E-mail: sgonias{at}ucsd.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Gaultier, S. Arandjelovic, S. Niessen, C. D. Overton, M. F. Linton, S. Fazio, W. M. Campana, B. F. Cravatt III, and S. L. Gonias Regulation of tumor necrosis factor receptor-1 and the IKK-NF-{kappa}B pathway by LDL receptor-related protein explains the antiinflammatory activity of this receptor Blood, June 1, 2008; 111(11): 5316 - 5325. [Abstract] [Full Text] [PDF]

				V. Montel, A. Gaultier, R. D. Lester, W. M. Campana, and S. L. Gonias The Low-Density Lipoprotein Receptor Related Protein Regulates Cancer Cell Survival and Metastasis Development Cancer Res., October 15, 2007; 67(20): 9817 - 9824. [Abstract] [Full Text] [PDF]

				X. Zhang, R. Polavarapu, H. She, Z. Mao, and M. Yepes Tissue-Type Plasminogen Activator and the Low-Density Lipoprotein Receptor-Related Protein Mediate Cerebral Ischemia-Induced Nuclear Factor-{kappa}B Pathway Activation Am. J. Pathol., October 1, 2007; 171(4): 1281 - 1290. [Abstract] [Full Text] [PDF]

				M. Padmasekar, R. Nandigama, M. Wartenberg, K.-D. Schluter, and H. Sauer The acute phase protein {alpha}2-macroglobulin induces rat ventricular cardiomyocyte hypertrophy via ERK1,2 and PI3-kinase/Akt pathways* Cardiovasc Res, July 1, 2007; 75(1): 118 - 128. [Abstract] [Full Text] [PDF]

				W. M. Campana, X. Li, N. Dragojlovic, J. Janes, A. Gaultier, and S. L. Gonias The Low-Density Lipoprotein Receptor-Related Protein Is a Pro-Survival Receptor in Schwann Cells: Possible Implications in Peripheral Nerve Injury J. Neurosci., October 25, 2006; 26(43): 11197 - 11207. [Abstract] [Full Text] [PDF]