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J. Biol. Chem., Vol. 281, Issue 11, 7374-7383, March 17, 2006

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Cyclin-dependent Kinase (CDK) Inhibitors Regulate the CDK-Cyclin Complex Activities in Endoreduplicating Cells of Developing Tomato Fruit^*

Badia Bisbis¹², Frédéric Delmas¹, Jérôme Joubès³, Adrien Sicard, Michel Hernould, Dirk Inzé, Armand Mouras, and Christian Chevalier⁴

From the Unité Mixte de Recherche 619 en Physiologie et Biotechnologie Végétales, Institut de Biologie Végétale Moléculaire, Institut National de la Recherche Agronomique, Université de Bordeaux 1 et Université Victor Segalen-Bordeaux 2, BP 81, 33883 Villenave d'Ornon Cedex, France and Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9052 Gent, Belgium

The jelly-like locular (gel) tissue of tomato fruit is made up of large thin-walled and highly vacuolized cells. The development of the gel tissue is characterized by the arrest of mitotic activities, the inhibition of cyclin-dependent kinase A (CDKA) activity, and numerous rounds of nuclear DNA endoreduplication. To decipher the molecular determinants controlling these developmental events, we investigated the putative involvement of CDK inhibitors (p27^Kip-related proteins, or KRPs) during the endoreduplication process. Two cDNAs, LeKRP1 and LeKRP2, encoding tomato CDK inhibitors were isolated. The LeKRP1 and LeKRP2 transcript expression was shown to be enhanced in the differentiating cells of the gel undergoing endoreduplication. At the translational level, LeKRP1 was shown to accumulate in the gel tissue and to participate in the inhibition of the CDK-cyclin kinase activities occurring in endoreduplicating cells of the gel tissue. We here propose that LeKRP1 participates in the control of both the cell cycle and the endoreduplication cycle.

Received for publication, June 17, 2005 , and in revised form, January 9, 2006.

The nucleotide sequence(s) reported in this paper has been submitted to the DDBJ/GenBank^TM/EBI Data Bank with accession number(s) AJ441249 [GenBank] and AJ441250 [GenBank] .

^* This work was supported in part by Grants 00-512858 and 10697-2003 from the Ministère de la Recherche et de la Technologie (France) (to F. D. and A. S., respectively) and by funding from the Region Aquitaine. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Both authors contributed equally to this work.

² Recipient of a postdoctoral fellowship from the Département de Biologie Végétale of Institut National de la Recherche Agonomique.

³ Recipient of a European Molecular Biology Organization fellowship. Present address: Laboratoire de Biogenèse Membranaire, CNRS FRE 2694, Université Victor Ségalen-Bordeaux 2, 146 Rue Léo Saignat-Case 92, 33076 Bordeaux Cedex, France.

⁴ To whom correspondence should be addressed. Tel./Fax: 33-557-122541; E-mail: chevalie{at}bordeaux.inra.fr.

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