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J. Biol. Chem., Vol. 281, Issue 13, 8339-8346, March 31, 2006

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Identification of the mstE Gene Encoding a Glucose-inducible, Low Affinity Glucose Transporter in Aspergillus nidulans^*

Josep V. Forment¹, Michel Flipphi², Daniel Ramón, Luisa Ventura, and Andrew P. MacCabe³

From the Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, Burjassot, 46100 Valencia, Spain and Departamento de Medicina Preventiva y Salud Pública, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universitat de València, Burjassot, 46100 Valencia, Spain

The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

Received for publication, July 27, 2005 , and in revised form, January 13, 2006.

^* This work has been supported by the European Union Grants BIO-4CT96-0535 and QLK3-CT99-00729 and the Generalitat Valenciana Grant GV05/099. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Recipient of a grant from the Ministerio de Educación, Cultura y Deporte.

² Recipient of a postdoctoral contract Ramón y Cajal from the Ministerio de Educación, Cultura y Deporte.

³ To whom correspondence should be addressed. Tel.: 34-963-900-022 (ext. 2309); Fax: 34-963-636-301; E-mail: andrew{at}iata.csic.es.

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