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J. Biol. Chem., Vol. 281, Issue 14, 9205-9209, April 7, 2006
The Role of Drosophila ninaG Oxidoreductase in Visual Pigment Chromophore Biogenesis*![]() ![]() ![]() 1
From the
We previously reported (Sarfare, S., Ahmad, S. T., Joyce, M. V., Boggess, B., and O'Tousa, J. E. (2005) J. Biol. Chem. 280, 1189511901) that the Drosophila ninaG gene encodes an oxidoreductase involved in the biosynthesis of the (3S)-3-hydroxyretinal serving as chromophore for Rh1 rhodopsin and that ninaG mutant flies expressing Rh4 as the major opsin accumulate large amounts of a different retinoid. Here, we show that this unknown retinoid is 11-cis-3-hydroxyretinol. Reversed phase high performance liquid chromatography coupled with a photodiode array UV-visible absorbance detector and mass spectrometer revealed a major product eluting at a retention time, tr, of 3.5 min with a
Received for publication, September 19, 2005 , and in revised form, December 29, 2005. * The work was supported by National Institutes of Health Grant EY06808. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 To whom correspondence should be addressed. Tel.: 574-631-6093; Fax: 574-631-7413; E-mail: jotousa{at}nd.edu.
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