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J. Biol. Chem., Vol. 281, Issue 14, 9351-9360, April 7, 2006

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Serial Analysis of Gene Expression in Mouse Uterus at the Implantation Site^*

Xing-Hong Ma, Shi-Jun Hu, Hua Ni, Yue-Chao Zhao, Zhen Tian, Ji-Long Liu, Gang Ren, Xiao-Huan Liang, Hao Yu, Ping Wan, and Zeng-Ming Yang¹

From the College of Life Science, Northeast Agricultural University, Harbin 150030, China and the Shanghai Huaguan Biochip Company, Shanghai 201203, China

Although oligonucleotide chips, cDNA microarrays, differential display reverse transcription-PCR, and other approaches have been used to screen implantation-related molecules, the mechanism by which embryo implantation occurs is still unknown. The aim of this study was to profile the differential gene expression between interimplantation site and implantation site in mouse uterus on day 5 of pregnancy by serial analysis of gene expression (SAGE). In our two SAGE libraries of 11-bp tags, the total numbers of tags sequenced were 48,121 for the interimplantation site and 50,227 for the implantation site. There were 1,039 tags specifically expressed at interimplantation site, and 1,252 tags specifically expressed at the implantation site. Based on the p value, there were 195 tags significantly up-regulated at the interimplantation site and 261 tags significantly up-regulated at the implantation site, of which 100 genes were single matched at the interimplantation site and 127 genes were single matched at the implantation site, respectively. By reverse transcription-PCR, the tag ratio between the implantation site and interimplantation site was verified on 14 significantly changed genes. Using in situ hybridization, 1810014L12Rik, Psmb5, Cd63, Npm1, Fads3, and Tagln2 were shown to be highly expressed at the implantation site compared with the interimplantation site. Compared with the interimplantation site, Ddx39 was strongly expressed in the subluminal stromal cells at the implantation site on day 5 of pregnancy. Ddx39 expression at the implantation site was specifically induced by active blastocysts. Additionally, Ddx39 expression was significantly up-regulated by estrogen in the ovariectomized mice. In our SAGE data, many implantation-related genes were identified in mouse uterus. Our data could be a valuable source for future study on embryo implantation.

Received for publication, October 24, 2005 , and in revised form, December 8, 2005.

^* This work was supported by Chinese National Natural Science Foundation Grants 30270163, 30330060, and 30570198 and Special Funds for Major State Basic Research Project Grant G1999055903. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Tables 4 and 5.

¹ To whom correspondence should be addressed: College of Life Sciences, Northeast Agricultural University, Harbin 150030, China. Tel.: 86-451-55191416; Fax: 86-451-55103336; E-mail: zmyang{at}neau.edu.cn.

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