HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 14, 9393-9399, April 7, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/14/9393    most recent M511975200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luo, Y. Articles by Panin, V. M. Search for Related Content PubMed PubMed Citation Articles by Luo, Y. Articles by Panin, V. M. Social Bookmarking                      What's this?

Protein O-Fucosyltransferase 2 Adds O-Fucose to Thrombospondin Type 1 Repeats^*

Yi Luo, Kate Koles, Wendy Vorndam, Robert S. Haltiwanger¹, and Vladislav M. Panin²

From the Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, New York 11794-5215 and the Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128

O-Fucose is an unusual form of glycosylation found on epidermal growth factor-like (EGF) repeats and thrombospondin type 1 repeats (TSRs) in many secreted and transmembrane proteins. Recently O-fucose on EGF repeats was shown to play important roles in Notch signaling. In contrast, physiological roles for O-fucose on TSRs are unknown. In the accompanying paper (Luo, Y., Nita-Lazar, A., and Haltiwanger, R. S. (2006) J. Biol. Chem. 281, 9385–9392), we demonstrated that an enzyme distinct from protein O-fucosyltransferase 1 adds O-fucose to TSRs. A known homologue of O-fucosyltransferase 1 is putative protein O-fucosyltransferase 2. The cDNA sequence encoding O-fucosyltransferase 2 was originally identified during a data base search for fucosyltransferases in Drosophila. Like O-fucosyltransferase 1, O-fucosyltransferase 2 is conserved from Caenorhabditis elegans to humans. Although O-fucosyltransferase 2 was assumed to be another protein O-fucosyltransferase, no biochemical characterization existed supporting this contention. Here we show that RNAi-mediated reduction of the O-fucosyltransferase 2 message significantly decreased TSR-specific O-fucosyltransferase activity in Drosophila S2 cells. We also found that O-fucosyltransferase 2 is predominantly localized in the endoplasmic reticulum compartment of these cells. Furthermore, we expressed recombinant Drosophila O-fucosyltransferase 2 and showed that it O-fucosylates TSRs but not EGF repeats in vitro. These results demonstrate that O-fucosyltransferase 2 is in fact a TSR-specific O-fucosyltransferase.

Received for publication, November 7, 2005 , and in revised form, February 7, 2006.

^* This work was supported in part by grants from the Mizutani Foundation for Glycosciences (to R. S. H.), the Carol M. Baldwin Breast Cancer Foundation (to R. S. H.), National Institutes of Health Grant GM 61126 (to R. S. H.), ARP/ATP Grant 000517-0069-2001 from the Texas Higher Education Coordinating Board and National Institutes of Health Grant GM069952 (to V. M. P.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence may be addressed. Tel.: 631-632-7336; Fax: 631-632-8575; E-mail: rhaltiwanger{at}ms.cc.sunysb.edu. ² To whom correspondence may be addressed. Tel.: 979-458-4630; Fax: 979-845-9274; E-mail: panin{at}tamu.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M. Gebauer, S. Muller, F.-G. Hanisch, M. Paulsson, and R. Wagener O-Glucosylation and O-Fucosylation Occur Together in Close Proximity on the First Epidermal Growth Factor Repeat of AMACO (VWA2 Protein) J. Biol. Chem., June 27, 2008; 283(26): 17846 - 17854. [Abstract] [Full Text] [PDF]

				D. Hess, J. J. Keusch, S. A. L. Oberstein, R. C. M. Hennekam, and J. Hofsteenge Peters Plus Syndrome Is a New Congenital Disorder of Glycosylation and Involves Defective O-Glycosylation of Thrombospondin Type 1 Repeats J. Biol. Chem., March 21, 2008; 283(12): 7354 - 7360. [Abstract] [Full Text] [PDF]

				H. Manya, T. Suzuki, K. Akasaka-Manya, H.-K. Ishida, M. Mizuno, Y. Suzuki, T. Inazu, N. Dohmae, and T. Endo Regulation of Mammalian Protein O-Mannosylation: PREFERENTIAL AMINO ACID SEQUENCE FOR O-MANNOSE MODIFICATION J. Biol. Chem., July 13, 2007; 282(28): 20200 - 20206. [Abstract] [Full Text] [PDF]

				S. Shi, C. Ge, Y. Luo, X. Hou, R. S. Haltiwanger, and P. Stanley The Threonine That Carries Fucose, but Not Fucose, Is Required for Cripto to Facilitate Nodal Signaling J. Biol. Chem., July 13, 2007; 282(28): 20133 - 20141. [Abstract] [Full Text] [PDF]

				L. M. Ricketts, M. Dlugosz, K. B. Luther, R. S. Haltiwanger, and E. M. Majerus O-Fucosylation Is Required for ADAMTS13 Secretion J. Biol. Chem., June 8, 2007; 282(23): 17014 - 17023. [Abstract] [Full Text] [PDF]

				L. W. Wang, M. Dlugosz, R. P. T. Somerville, M. Raed, R. S. Haltiwanger, and S. S. Apte O-Fucosylation of Thrombospondin Type 1 Repeats in ADAMTS-like-1/Punctin-1 Regulates Secretion: IMPLICATIONS FOR THE ADAMTS SUPERFAMILY J. Biol. Chem., June 8, 2007; 282(23): 17024 - 17031. [Abstract] [Full Text] [PDF]

				K. Kozma, J. J. Keusch, B. Hegemann, K. B. Luther, D. Klein, D. Hess, R. S. Haltiwanger, and J. Hofsteenge Identification and Characterization of abeta1,3-Glucosyltransferase That Synthesizes the Glc-beta1,3-Fuc Disaccharide on Thrombospondin Type 1 Repeats J. Biol. Chem., December 1, 2006; 281(48): 36742 - 36751. [Abstract] [Full Text] [PDF]

				T. Sato, M. Sato, K. Kiyohara, M. Sogabe, T. Shikanai, N. Kikuchi, A. Togayachi, H. Ishida, H. Ito, A. Kameyama, et al. Molecular cloning and characterization of a novel human {beta}1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain Glycobiology, December 1, 2006; 16(12): 1194 - 1206. [Abstract] [Full Text] [PDF]

				B. Ma, J. L. Simala-Grant, and D. E. Taylor Fucosylation in prokaryotes and eukaryotes Glycobiology, December 1, 2006; 16(12): 158R - 184R. [Abstract] [Full Text] [PDF]

				C. Loriol, F. Dupuy, R. Rampal, M.A. Dlugosz, R.S. Haltiwanger, A. Maftah, and A. Germot Molecular evolution of protein O-fucosyltransferase genes and splice variants Glycobiology, August 1, 2006; 16(8): 736 - 747. [Abstract] [Full Text] [PDF]