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Originally published In Press as doi:10.1074/jbc.M508846200 on February 3, 2006
J. Biol. Chem., Vol. 281, Issue 15, 10035-10041, April 14, 2006
Mechanistic Basis for the Action of New Cephalosporin Antibiotics Effective against Methicillin- and Vancomycin-resistant Staphylococcus aureus*
Cosimo Fuda ,
Dusan Hesek ,
Mijoon Lee ,
Werner Heilmayer ,
Rodger Novak ,
Sergei B. Vakulenko , and
Shahriar Mobashery 1
From the
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 and Antibiotic Research Institute, Sandoz GmbH, Brunner Strasse, A-1235 Vienna, Austria
Emergence of methicillin-resistant Staphylococcus aureus (MRSA) has created challenges in treatment of nosocomial infections. The recent clinical emergence of vancomycin-resistant MRSA is a new disconcerting chapter in the evolution of these strains. S. aureus normally produces four PBPs, which are susceptible to modification by -lactam antibiotics, an event that leads to bacterial death. The gene product of mecA from MRSA is a penicillin-binding protein (PBP) designated PBP 2a. PBP 2a is refractory to the action of all commercially available -lactam antibiotics. Furthermore, PBP 2a is capable of taking over the functions of the other PBPs of S. aureus in the face of the challenge by -lactam antibiotics. Three cephalosporins (compounds 13) have been studied herein, which show antibacterial activities against MRSA, including the clinically important vancomycin-resistant strains. These cephalosporins exhibit substantially smaller dissociation constants for the preacylation complex compared with the case of typical cephalosporins, but their pseudo-second-order rate constants for encounter with PBP 2a (k2/Ks) are not very large ( 200 M1 s1). It is documented herein that these cephalosporins facilitate a conformational change in PBP 2a, a process that is enhanced in the presence of a synthetic surrogate for cell wall, resulting in increases in the k2/Ks parameter and in more facile enzyme inhibition. These findings argue that the novel cephalosporins are able to co-opt interactions between PBP 2a and the cell wall in gaining access to the active site in the inhibition process, a set of events that leads to effective inhibition of PBP 2a and the attendant killing of the MRSA strains.
Received for publication, August 10, 2005
, and in revised form, February 3, 2006.
* This research was supported by a grant from the National Institutes of Health (NIH). The Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) program is supported by NIAID, NIH, Contract NO1-AI-95359. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 To whom correspondence should be addressed: Dept. of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556. Tel.: 574-631-6652; Fax: 574-631-2933; E-mail: mobashery{at}nd.edu.

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Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
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