HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 16, 10839-10848, April 21, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/16/10839    most recent M508858200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Metz, R. P. Articles by Porter, W. W. Search for Related Content PubMed PubMed Citation Articles by Metz, R. P. Articles by Porter, W. W. Social Bookmarking                      What's this?

Differential Transcriptional Regulation by Mouse Single-minded 2s^*

From the Department of Integrated Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4458

Single-minded 1 and 2 are unique members of the basic helix-loop-helix Per-Arnt-Sim family as they are transcriptional repressors. Here we report the identification and transcriptional characterization of mouse Sim2s, a splice variant of Sim2, which is missing the carboxyl Pro/Ala-rich repressive domain. Sim2s is expressed at high levels in kidney and skeletal muscle; however, the ratio of Sim2 to Sim2s mRNA differs between these tissues. Similar to full-length Sim2, Sim2s interacts with Arnt and to a lesser extent, Arnt2. The effects of Sim2s on transcriptional regulation through hypoxia, dioxin, and central midline response elements are different than that of full-length Sim2. Specifically, Sim2s exerts a less repressive effect on hypoxia-induced gene expression than full-length Sim2, but is just as effective as Sim2 at repressing TCDD-induced gene expression from a dioxin response element. Interestingly, Sim2s bind to and activates expression from a central midline response element-controlled reporter through an Arnt transactivation domain-dependent mechanism. The differences in expression pattern, protein interactions, and transcriptional activities between Sim2 and Sim2s may reflect differential roles each isoform plays during development or in tissue-specific effects on other protein-mediated pathways.

Received for publication, August 11, 2005 , and in revised form, January 12, 2006.

^* This work was supported by National Institutes of Health NCI Grant RO1CA111551 (to W. W. P.), a Howard Hughes Predoctoral Fellowship (to T. G.), and National Institutes of Health NIEHS Pilot Project P30ES09106 (to W. W. P.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom corresponding should be addressed: MS 4458 Texas A&M University, College Station, TX 77843-4458. Tel.: 979-845-0733; Fax: 979-862-4929; E-mail: wporter{at}cvm.tamu.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Woods, A. Farrall, C. Procko, and M. L. Whitelaw The bHLH/Per-Arnt-Sim transcription factor SIM2 regulates muscle transcript myomesin2 via a novel, non-canonical E-box sequence Nucleic Acids Res., June 1, 2008; 36(11): 3716 - 3727. [Abstract] [Full Text] [PDF]

				B. Laffin, E. Wellberg, H.-I. Kwak, R. C. Burghardt, R. P. Metz, T. Gustafson, P. Schedin, and W. W. Porter Loss of Singleminded-2s in the Mouse Mammary Gland Induces an Epithelial-Mesenchymal Transition Associated with Up-Regulation of Slug and Matrix Metalloprotease 2 Mol. Cell. Biol., March 15, 2008; 28(6): 1936 - 1946. [Abstract] [Full Text] [PDF]

				H.-I. Kwak, T. Gustafson, R. P. Metz, B. Laffin, P. Schedin, and W. W. Porter Inhibition of breast cancer growth and invasion by single-minded 2s Carcinogenesis, February 1, 2007; 28(2): 259 - 266. [Abstract] [Full Text] [PDF]

				O. J. Halvorsen, K. Rostad, A. M. Oyan, H. Puntervoll, T. H. Bo, L. Stordrange, S. Olsen, S. A. Haukaas, L. Hood, I. Jonassen, et al. Increased Expression of SIM2-s Protein Is a Novel Marker of Aggressive Prostate Cancer Clin. Cancer Res., February 1, 2007; 13(3): 892 - 897. [Abstract] [Full Text] [PDF]