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J. Biol. Chem., Vol. 281, Issue 18, 12248-12252, May 5, 2006

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Subunit a of Cytochrome o Oxidase Requires Both YidC and SecYEG for Membrane Insertion^*

From the Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands

The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E. coli leads to a specific defect in the functional assembly of major energy transducing complexes such as the F₁F₀ ATPase and cytochrome bo₃ oxidase. Here we report on the in vitro reconstitution of the membrane insertion of the CyoA subunit of cytochrome bo₃ oxidase. Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force. These data demonstrate that pre-CyoA is a substrate of a novel pathway that involves both SecYEG and YidC.

Received for publication, January 3, 2006 , and in revised form, February 9, 2006.

^* This work was supported by the Netherlands Royal Academy of Sciences. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel. 31-50-632164; Fax: 31-50-3632154; E-mail: a.j.m.driessen{at}rug.nl.

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