HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 18, 12776-12785, May 5, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/18/12776    most recent M512769200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sarkar, M. Articles by Boulianne, G. L. Search for Related Content PubMed PubMed Citation Articles by Sarkar, M. Articles by Boulianne, G. L. Social Bookmarking                      What's this?

Null Mutations in Drosophila N-Acetylglucosaminyltransferase I Produce Defects in Locomotion and a Reduced Life Span^*

Mohan Sarkar, Peter A. Leventis^¶, Cristina I. Silvescu^||, Vernon N. Reinhold^||, Harry Schachter^**1, and Gabrielle L. Boulianne^¶2

From the Program in Structural Biology and Biochemistry and the Program in Developmental Biology, The Hospital for Sick Ontario M5G 1X8, Canada, the ^¶Department of Zoology, University of Toronto, Toronto, Ontario M5S 3G5, Canada, the ^||Department of Chemistry, the University of New Hampshire, Durham, New Hampshire 03824, and the ^**Department of Biochemistry and the Department of Molecular and Medical Genetics, the University of Toronto, Toronto, Ontario M5S 1A8, Canada

UDP-GlcNAc:3-D-mannoside 1,2-N-acetylglucosaminyltransferase I (encoded by Mgat1) controls the synthesis of hybrid, complex, and paucimannose N-glycans. Mice make hybrid and complex N-glycans but little or no paucimannose N-glycans. In contrast, Drosophila melanogaster and Caenorhabditis elegans make paucimannose N-glycans but little or no hybrid or complex N-glycans. To determine the functional requirement for 1,2-N-acetylglucosaminyltransferase I in Drosophila, we generated null mutations by imprecise excision of a nearby transposable element. Extracts from Mgat1¹/Mgat1¹ null mutants showed no 1,2-N-acetylglucosaminyltransferase I enzyme activity. Moreover, mass spectrometric analysis of these extracts showed dramatic changes in N-glycans compatible with lack of 1,2-N-acetylglucosaminyltransferase I activity. Interestingly, Mgat1¹/Mgat1¹ null mutants are viable but exhibit pronounced defects in adult locomotory activity when compared with Mgat1¹/CyO-GFP heterozygotes or wild type flies. In addition, in null mutants males are sterile and have a severely reduced mean and maximum life span. Microscopic examination of mutant adult fly brains showed the presence of fused lobes. The removal of both maternal and zygotic Mgat1 also gave rise to embryos that no longer express the horseradish peroxidase antigen within the central nervous system. Taken together, the data indicate that 1,2-N-acetylglucosaminyltransferase I-dependent N-glycans are required for locomotory activity, life span, and brain development in Drosophila.

Received for publication, November 29, 2005 , and in revised form, February 14, 2006.

^* This work was supported by funds from the Canadian Institutes of Health Research (to H. S. and G. L. B.), Natural Science and Engineering Research Council funds (to G. L. B.), and a Canadian Institutes of Health Research doctoral research award (to P. A. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

² Recipient of a Canada Research Chair in Molecular and Developmental Neurobiology.

¹ To whom correspondence should be addressed: Program in Structural Biology and Biochemistry, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada. Tel.: 416-813-5915; Fax: 416-813-5022; E-mail: harry{at}sickkids.ca.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. G. T. Hagen, L. Zhang, E Tian, and Y. Zhang Glycobiology on the fly: Developmental and mechanistic insights from Drosophila Glycobiology, February 1, 2009; 19(2): 102 - 111. [Abstract] [Full Text] [PDF]

				K. S Lau and J. W Dennis N-Glycans in cancer progression Glycobiology, October 1, 2008; 18(10): 750 - 760. [Abstract] [Full Text] [PDF]

				R. B. Zavareh, K. S. Lau, R. Hurren, A. Datti, D. J. Ashline, M. Gronda, P. Cheung, C. D. Simpson, W. Liu, A. R. Wasylishen, et al. Inhibition of the Sodium/Potassium ATPase Impairs N-Glycan Expression and Function Cancer Res., August 15, 2008; 68(16): 6688 - 6697. [Abstract] [Full Text] [PDF]

				J. M. Prien, L. C. Huysentruyt, D. J. Ashline, A. J. Lapadula, T. N. Seyfried, and V. N. Reinhold Differentiating N-linked glycan structural isomers in metastatic and nonmetastatic tumor cells using sequential mass spectrometry Glycobiology, May 1, 2008; 18(5): 353 - 366. [Abstract] [Full Text] [PDF]

				J. S. Kang, J. Frank, C. H. Kang, H. Kajiura, M. Vikram, A. Ueda, S. Kim, J. D. Bahk, B. Triplett, K. Fujiyama, et al. Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus PNAS, April 15, 2008; 105(15): 5933 - 5938. [Abstract] [Full Text] [PDF]

				K. Koles, J.-M. Lim, K. Aoki, M. Porterfield, M. Tiemeyer, L. Wells, and V. Panin Identification of N-Glycosylated Proteins from the Central Nervous System of Drosophila Melanogaster Glycobiology, December 1, 2007; 17(12): 1388 - 1403. [Abstract] [Full Text] [PDF]

				M. Gutternigg, D. Kretschmer-Lubich, K. Paschinger, D. Rendic, J. Hader, P. Geier, R. Ranftl, V. Jantsch, G. Lochnit, and I. B. H. Wilson Biosynthesis of Truncated N-Linked Oligosaccharides Results from Non-orthologous Hexosaminidase-mediated Mechanisms in Nematodes, Plants, and Insects J. Biol. Chem., September 21, 2007; 282(38): 27825 - 27840. [Abstract] [Full Text] [PDF]

				K. Nguyen, I. van Die, K. M Grundahl, Z. S Kawar, and R. D Cummings Molecular cloning and characterization of the Caenorhabditis elegans {alpha}1,3-fucosyltransferase family Glycobiology, June 1, 2007; 17(6): 586 - 599. [Abstract] [Full Text] [PDF]

				K. Aoki, M. Perlman, J.-M. Lim, R. Cantu, L. Wells, and M. Tiemeyer Dynamic Developmental Elaboration of N-Linked Glycan Complexity in the Drosophila melanogaster Embryo J. Biol. Chem., March 23, 2007; 282(12): 9127 - 9142. [Abstract] [Full Text] [PDF]

				T. Ju, Q. Zheng, and R. D. Cummings Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans Glycobiology, October 1, 2006; 16(10): 947 - 958. [Abstract] [Full Text] [PDF]

				M. Crispin, D. J. Harvey, V. T. Chang, C. Yu, A. R. Aricescu, E. Y. Jones, S. J. Davis, R. A. Dwek, and P. M. Rudd Inhibition of hybrid- and complex-type glycosylation reveals the presence of the GlcNAc transferase I-independent fucosylation pathway Glycobiology, August 1, 2006; 16(8): 748 - 756. [Abstract] [Full Text] [PDF]