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J. Biol. Chem., Vol. 281, Issue 19, 13030-13037, May 12, 2006

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Silencing the Activity and Proliferative Properties of the Human EagI Potassium Channel by RNA Interference^*

From the Max Planck Institute of Experimental Medicine, Hermann-Rein-Str. 3, 37075 Göttingen, Germany

EagI potassium channels are natively expressed in the mammalian brain as well as in many cancer cell lines and tumor tissues. The role of EagI in malignant transformation has been suggested by several experiments, but the lack of specific EagI inhibitors has made it difficult to examine the influence of the channel on oncogenesis and its potential as a therapeutic target. We have used short interfering RNA to test the effects of EagI reduction on the behavior of tumor cells in vitro. By generating and optimizing an EagI-specific short interfering RNA system, we were able to study the effects of EagI depletion on several cancer cell lines that endogenously express this protein. We show here that our short interfering RNA sequences act specifically on EagI, reproducibly induce a significant decrease in the proliferation of tumor cell lines, and do not trigger any observable nonspecific responses.

Received for publication, January 30, 2006 , and in revised form, March 2, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Max Planck Institute of Experimental Medicine, Dept. of Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany. Tel.: 49-551-3899643; Fax: 49-551-3899644; E-mail: Pardo{at}em.mpg.de.

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