Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M511590200 on March 14, 2006

J. Biol. Chem., Vol. 281, Issue 19, 13817-13827, May 12, 2006
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
281/19/13817    most recent
M511590200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanematsu, F.
Right arrow Articles by Nakayama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanematsu, F.
Right arrow Articles by Nakayama, T.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Asf1 Is Required for Viability and Chromatin Assembly during DNA Replication in Vertebrate Cells*

Fumiyuki Sanematsu{ddagger}, Yasunari Takami{ddagger}, Hirak Kumar Barman{ddagger}, Tatsuo Fukagawa§, Tatsuya Ono, Kei-ichi Shibahara, and Tatsuo Nakayama{ddagger}||1

From the {ddagger}Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan, the Department of Integrated Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan, the §Department of Molecular Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan, and the ||Department of Life Science, Frontier Science Research Center, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan

Asf1 (anti-silencing function 1), a well conserved protein from yeast to humans, acts as a histone chaperone and is predicted to participate in a variety of chromatin-mediated cellular processes. To investigate the physiological role of vertebrate Asf1 in vivo, we generated a conditional Asf1-deficient mutant from chicken DT40 cells. Induction of Asf1 depletion resulted in the accumulation of cells in S phase with decreased DNA replication and increased mitotic aberrancy forming multipolar spindles, leading to cell death. In addition, nascent chromatin in Asf1-depleted cells showed increased nuclease sensitivity, indicating impaired nucleosome assembly during DNA replication. Complementation analyses revealed that the functional domain of Asf1 for cell viability was confined to the N-terminal core domain (amino acids 1-155) that is a binding platform for histones H3/H4, CAF-1p60, and HIRA, whereas Asf1 mutant proteins, abolishing binding abilities with both p60 and HIRA, exhibit no effect on viability. These results together indicate that the vertebrate Asf1 plays a crucial role in replication-coupled chromatin assembly, cell cycle progression, and cellular viability and provide a clue of a possible role in a CAF-1- and HIRA-independent chromatin-modulating process for cell proliferation.


Received for publication, October 26, 2005 , and in revised form, March 14, 2006.

* This work was supported in part by the 21st Century Center of Excellence Program (Life Science); a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology; and grants from the Japan Society for the Promotion of Science and CREST from Japan Science and Technology of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 To whom correspondence should be addressed: Section of Biochemistry and Molecular Biology, Dept. of Medical Sciences, Miyazaki Medical College, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan. Tel.: 81-985-85-3127; Fax: 81-985-85-6503; E-mail: tnakayam{at}med.miyazaki-u.ac.jp.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Virol.Home page
A. P. Ambagala, T. Bosma, M. A. Ali, M. Poustovoitov, J. J. Chen, M. D. Gershon, P. D. Adams, and J. I. Cohen
Varicella-Zoster Virus Immediate-Early 63 Protein Interacts with Human Antisilencing Function 1 Protein and Alters Its Ability To Bind Histones H3.1 and H3.3
J. Virol., January 1, 2009; 83(1): 200 - 209.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
A. Galvani, R. Courbeyrette, M. Agez, F. Ochsenbein, C. Mann, and J.-Y. Thuret
In Vivo Study of the Nucleosome Assembly Functions of ASF1 Histone Chaperones in Human Cells
Mol. Cell. Biol., June 1, 2008; 28(11): 3672 - 3685.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
A. Groth, A. Corpet, A. J. L. Cook, D. Roche, J. Bartek, J. Lukas, and G. Almouzni
Regulation of Replication Fork Progression Through Histone Supply and Demand
Science, December 21, 2007; 318(5858): 1928 - 1931.
[Abstract] [Full Text] [PDF]


Home page
GENES CELLSHome page
M. Nakayama, H. Suzuki, N. Yamamoto-Nagamatsu, H. K. Barman, H. Kikuchi, Y. Takami, K. Toyonaga, K. Yamashita, and T. Nakayama
HDAC2 controls IgM H- and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions
Genes Cells, March 1, 2007; 12(3): 359 - 373.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
Y. Takami, T. Ono, T. Fukagawa, K.-i. Shibahara, and T. Nakayama
Essential Role of Chromatin Assembly Factor-1-mediated Rapid Nucleosome Assembly for DNA Replication and Cell Division in Vertebrate Cells
Mol. Biol. Cell, January 1, 2007; 18(1): 129 - 141.
[Abstract] [Full Text] [PDF]


Home page
Eukaryot CellHome page
J. Linger and J. K. Tyler
Global Replication-Independent Histone H4 Exchange in Budding Yeast
Eukaryot. Cell, October 1, 2006; 5(10): 1780 - 1787.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement