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J. Biol. Chem., Vol. 281, Issue 2, 800-806, January 13, 2006

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A Splicing Repressor Domain in Polypyrimidine Tract-binding Protein^*

From the Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom

Polypyrimidine tract-binding protein (PTB) is an hnRNP with four RRM type domains. It plays roles as a repressive alternative splicing regulator of multilple target genes, as well as being involved in pre-mRNA 3' end processing, mRNA localization, stability, and internal ribosome entry site-mediated translation. Here we have used a tethered function assay, in which a fusion protein of PTB and the bacteriophage MS2 coat protein is recruited to a splicing regulatory site by binding to an artificially inserted MS2 binding site. Deletion mutations of PTB in this system allowed us to identify RRM2 and the following inter-RRM linker region as the minimal region of PTB that can act as splicing repressor domain when recruited to RNA. Splicing repression by the minimal repressor domain remained cell type-specific and dependent upon other defined regulatory elements in the -tropomyosin test minigene. Our results highlight the fact that splicing repression by PTB can be uncoupled from the mode by which it binds to RNA.

Received for publication, September 27, 2005 , and in revised form, November 4, 2005.

^* This work was supported in part by Wellcome Trust Program Grant 059879. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Supported by studentship funds from the Canadian Cambridge Trust with Merck Frosst Canada Inc., an Overseas Research Studentship, Cambridge Commonwealth Trust, and by Corpus Christi College. Current address: Depts. of Developmental Biology and Cancer Research, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.

² To whom correspondence should be addressed. Fax: 44-1223-766002; E-mail: cwjs1{at}cam.ac.uk.

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				C. Wang, J. T. Norton, S. Ghosh, J. Kim, K. Fushimi, J. Y. Wu, M. S. Stack, and S. Huang Polypyrimidine Tract-binding Protein (PTB) Differentially Affects Malignancy in a Cell Line-dependent Manner J. Biol. Chem., July 18, 2008; 283(29): 20277 - 20287. [Abstract] [Full Text] [PDF]

				A. J. Matlin, J. Southby, C. Gooding, and C. W.J. Smith Repression of {alpha}-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract RNA, August 1, 2007; 13(8): 1214 - 1223. [Abstract] [Full Text] [PDF]